Hello everyone,

I have a technical question abou extracellular vesicles. I harvest conditioned medium from a bioreactor in which the cells are seeded in medium without serum. Then I run the sample through a tangentional flow filter (TFF) until my sample is concentrated to 1 mL in HEPES buffer. Next I store the samples in -80oC. Before doing the Western Blot should I add RIPA buffer to my EVs concentrated in HEPES? If yes, what is the right ratio sample : RIPA buffer?

Thank you in advance,

Karyna.