Dear all,
Could someone share/suggest a good protocol for preparing small unilamellar vesicles (SUVs) from multilamellar vesicles. Without using an Extruder.
Thanks in advance
There is no need to use harsh treatments such as sonication or homogenisation. As a mild and robust technique for the preparation of different types of lipid vesicles you can use "Mozafari Method" as described in the following publications:
1. Entrapment of rosemary extract by liposomes formulated by Mozafari method: physicochemical characterization and optimization
S Jahanfar, M Gahavami, K Khosravi-Darani, M Jahadi, MR Mozafari
Heliyon, e08632
Article Entrapment of Rosemary extract by liposomes formulated by Mo...
2. Potential micro-/nano-encapsulation systems for improving stability and bioavailability of anthocyanins: An updated review
AK Rashwan, N Karim, Y Xu, J Xie, H Cui, MR Mozafari, W Chen
Critical Reviews in Food Science and Nutrition, 1-24
3. Recent Trends in the Nanoencapsulation Processes for Food and Nutraceutical Applications
S Khorasani, APG Yazdi, E Taghavi, MAA Abadi, H Ghobadi, B Zihayat, ...
Innovative Food Processing Technologies: A Comprehensive Review. 3, 532–545 (Elsevier)
4. Nanoliposomes and tocosomes as multifunctional nanocarriers for the encapsulation of nutraceutical and dietary molecules
A Zarrabi, M Alipoor Amro Abadi, S Khorasani, MR Mohammadabadi, ...
Molecules 25 (3), 638
Article Nanoliposomes and Tocosomes as Multifunctional Nanocarriers ...
5. Enhanced efficacy and bioavailability of thymoquinone using nanoliposomal dosage form
MR Mohammadabadi, MR Mozafari
Journal of Drug Delivery Science and Technology 47, 445-453
Article Enhanced efficacy and bioavailability of thymoquinone using ...
To make SUV's, you will need a method other than extruding. You can either use a probe sonicator or french press.
Hi,
I use probe sonicator and RSM to optimize the result of the developed SUV. They will sort and arrange different concentration of your SUV composition and processing factors (sonication time, temperature, sonication power) so that the outcome is more consistent and you will get the optimum ratio to create your suv. Previously i used extruder but the sample amount is only small (1ml) hence i changed to probe sonicator.
Reference paper :
Surface-engineered liposomes for dual-drug delivery targeting strategy against methicillin-resistant Staphylococcus aureus (MRSA)
https://www.sciencedirect.com/science/article/pii/S1818087621001057
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