Protocol for RNA extraction from Transwell inserts?

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Hello, I have to work with cells HSC-3. Can you recommend me how to do polarized layers of this cells. Or maybe an article where it is good described?

Maybe exists protocol for this process. Thank you very much.

15 September 2022 6,937 0 View

Should I add a Lysis buffer (such as RIPA buffer) to Extracellular vesicles already in HEPES buffer before doing Western Blot?

Hello everyone, I have a technical question abou extracellular vesicles. I harvest conditioned medium from a bioreactor in which the cells are seeded in medium without serum. Then I run the...

31 July 2022 8,242 0 View

Protocol for SUV formation from MLV vesicles?

Dear all, Could someone share/suggest a good protocol for preparing small unilamellar vesicles (SUVs) from multilamellar vesicles. Without using an Extruder. Thanks in advance

07 March 2022 5,080 5 View

Can anyone help me with a protocol on how to manually design primers for PCR?

I have to manually design my primers for PCR assay. Can anyone give me more information on how to do it? Thank you in advance.

23 January 2022 5,355 8 View

What can it be? Bacillus? Proteus?

Has anyone seen anything like this growth? It was streak inoculation, The colony surface is dry, but inside is wet, the odor is putrefactive.

17 July 2016 7,978 26 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

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06 August 2024 1,367 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

Kashif Ameer

Karyna Tarasova

Go to this

https://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-55809/protocols/

Refer to methods P-GSE55809-2, 3 and 4 for nucleic acid extractions from Transwell inserts

Chinaza Godswill Awuchi

Check http://erj.ersjournals.com/highwire/filestream/127684/field_highwire_adjunct_files/1/ERJ-01282-2015_Suppl_Methods.pdf

https://www.stemcell.com/protocol-for-total-rna-isolation-from-cells.html

Check https://medicine.yale.edu/keck/ycga/Images/TRIZOLRNAIsolation_092107_tcm240-21453.pdf

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6085293/

Kiprotich Kiptum

Check on this paper

http://erj.ersjournals.com/highwire/filestream/127684/field_highwire_adjunct_files/1/ERJ-01282-2015_Suppl_Methods.pdf