Maybe exists protocol for this process. Thank you very much.
Hello everyone, I have a technical question abou extracellular vesicles. I harvest conditioned medium from a bioreactor in which the cells are seeded in medium without serum. Then I run the...
31 July 2022 8,242 0 View
Dear all, Could someone share/suggest a good protocol for preparing small unilamellar vesicles (SUVs) from multilamellar vesicles. Without using an Extruder. Thanks in advance
07 March 2022 5,080 5 View
I have to manually design my primers for PCR assay. Can anyone give me more information on how to do it? Thank you in advance.
23 January 2022 5,355 8 View
Can anyone hel me with a direct protocol to extract RNA from a 0.4 μm pore polyester membrane insert in which the membrane diam is 12 mm?
05 January 2022 7,589 4 View
I'm looking for the best methodology for calculating the CDI and the USI.
01 January 1970 1,387 2 View
If you think of electrons with spin as bar magnets, you know bar magnets of opposite polarity as long as they're not occupying the same spatial location don't cancel out each other's magnetic...
03 August 2024 3,927 2 View
Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?
22 July 2024 1,210 2 View
I am new to gaussian 16 software and while doing non linear optical properties calculation hyperpolarizabiltiy polarizability dipole moment calculation I found different gaussian input keywords...
08 July 2024 7,551 1 View
R(t) = the propagation function of the vortex, where R=sqrt(4vt + R0^2) R0 = initial radius. w0 = initial angular velocity. Rf = the radius of the boundary confining the fluid. The Lamb-Oseen...
07 July 2024 3,680 0 View
bodipy moiety are high polar nature .so its are bottom of TLC PALTE up to 20;80(ethyl acetate;hexane) mixture so its binding in SiO2 SO YIELD is so decreased. how to remove DDQ from reaction mixture?
02 July 2024 8,846 4 View
In the molecular dynamics simulation analysis part, how to calculate molecular surface area (MolSA) and polar surface area (PSA) in gromacs ?
14 June 2024 1,469 1 View
Polar ice
12 June 2024 9,997 1 View
I have read a few papers that have indicated that the sampling rate of the Polar h10 is 1000 Hz. 1) Navalta et al., Heart rate processing algorithms and exercise duration on reliability and...
10 June 2024 4,744 1 View
Give a concrete example to prove this
06 June 2024 3,630 0 View
Hello! I am trying to stain primary endothelial cells that have been grown on Matrigel to form tubules. I am interested in apical-basal polarity. The cells do form tubules but it is hard to image...
28 May 2024 5,855 4 View