Karyna Tarasova

5 Questions 2 Answers 0 Followers

Questions related from Karyna Tarasova

Should I add a Lysis buffer (such as RIPA buffer) to Extracellular vesicles already in HEPES buffer before doing Western Blot?

Hello everyone, I have a technical question abou extracellular vesicles. I harvest conditioned medium from a bioreactor in which the cells are seeded in medium without serum. Then I run the...

01 August 2022 8,253 0 View

What is the eccentricity values for MSCs cells?

Hello everyone, I am working now with a device called Incucyte to evaluate proliferation and to analyze migration and wound healing. Has anyone worked with this before? Could you please help me...

12 July 2022 5,803 0 View

Protocol for SUV formation from MLV vesicles?

Dear all, Could someone share/suggest a good protocol for preparing small unilamellar vesicles (SUVs) from multilamellar vesicles. Without using an Extruder. Thanks in advance

08 March 2022 5,105 5 View

Can anyone help me with a protocol on how to manually design primers for PCR?

I have to manually design my primers for PCR assay. Can anyone give me more information on how to do it? Thank you in advance.

24 January 2022 5,375 8 View

Protocol for RNA extraction from Transwell inserts?

Can anyone hel me with a direct protocol to extract RNA from a 0.4 μm pore polyester membrane insert in which the membrane diam is 12 mm?

06 January 2022 7,599 4 View