Hello,
I am currently working through a set of saliva samples for DNA extraction.
We have been following a protocol that begins by combining the sample with 60 ul of Proteinase K and 300 ul of 10% SDS, followed by an overnight incubation at 53°C. After the incubation we proceed directly into the rest of the procedure (addition of NaCl, centrifugation, isopropanol, centrifugation, ethanol, dissolve pellet in H2O, etc.).
My question is, as it is Friday, would it be alright to incubate the samples at that temperature for the duration of the weekend? Would this damage the samples? Alternatively, would it be okay to incubate the samples for approximately one hour, and then remove the samples and store at -20 for the weekend? Would this lower the DNA yield?
Thank you!
Julien
Hi dear
If you reduce the time of first incubation lysis of your samples will decrease so you have been less dna and more protein so it is better dont do that.
If you started first step and incubated for one hour you can freez them at -20 c degree and go on the incubation for overnight later or increase the incobation time more than an overnight but some of your dna can be broken.
It depends on your goal for extraction dna.
I will hope it be useful.
Store the samples at RT for short-term storage,4 °C for long-term storage (>3 months).or 20C for as long as you wish like Ali emphasized
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Biomolecules
- Nucleic Acids
- DNA