What company provide humanized mice?

21 February 2023 0 3K Report

Hi,

I need humanized mouse models for flavivirus infection but I do not have the skills to produce them. Do you know of a company that can do it.

Thank you very much

Badges
Science method

More Julien Pompon's questions See All

Sodium azide in immunohistochemistry?

Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...

23 July 2024 6,314 3 View

Increasing Area Ratio on Quality control Standard on LCMS ?

Hello everyone. During my analyses on LC MSMS, the area ratio (Standard Area/Isotopically labelled Standard Area) increases with time for the quality control point. I'm having trouble explaining...

08 July 2024 3,150 2 View

Systematic reviews and/or meta-analysis and articles from predatory journals/publishers ?

Do you know systematic reviews and/or meta-analyses that have chosen to exclude articles from (or suspected to be) predatory journals/publishers in their work ?

24 March 2024 4,941 3 View

How to store insect sf9 cells?

Is it possible to keep them in -80 freezer or should we keep them in liquid nitrogen?

17 July 2023 2,846 1 View

We're looking for a complete system for producing anodized aluminum oxide (AAO) substrates from self-supported aluminum foil?

12 June 2023 3,526 2 View

Is there any free AI tool (machine learning) for the prediction of urinary tract infection based on dipstiks and sediment?

Dear members, As you know, the identification of urinary tract infection by culture is crucial for the diagnosis and treatment. However, traditional methods of culture identification can be...

04 May 2023 7,178 3 View

No melting peak in DTA for an amorphous material ?

Hello everyone, I'm currently doing some DTA analysis on two phases of the same ceramic material, one is fully crystalline and the other fully amorphous. When I work with the crystalline one I...

03 July 2022 8,061 3 View

How to avoid yeast zygot clumping?

I would like to perform some microscopy experiments on yeast zygots. Unfortunetly zygots are clumping together which is not ideal condition for my experiment. I tried to add 0.1% of Tween80 with...

01 March 2022 8,606 2 View

Quantifying the uncertainty around the prediction of a logistic regression model: an available R package to estimate profile likelihood 95%CI?

Hi, I'm a fish biologist and I'm interested in assessing the uncertainty around the L50, which is the (sex-specific) length (L) at which you expect 1 fish out of 2 (50%) to exhibit developed...

21 November 2021 173 11 View

How to assess the phase stability of an spectrum in time?

Hi! I am working on a spectrometer-based OCT system and I would like to evaluate the phase stability of my interference spectra in time. How would you proceed?

16 September 2021 6,484 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View