Ripa lysis buffer recipe

Ibrahim Khaleelullah Baig @Ibrahim-Khaleelullah-Baig

01 January 1970 1 214 Report

How much microlitre of Phosphatase inhibitors should be added to the ripa lysis buffer ? The working range is 1:100.How much I should add for 500ul of RIPA?

Nimisha Pradeep George

I would say 5ul Protease inhibitor in 495ul RIPA buffer (1:100 dilution).

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Science method

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