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Questions related from Ibrahim Khaleelullah Baig
What does conditional and constitutional activation of kinases refers to
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I did double digestion for ERBB2 Wild type plasmid. I followed NEB protocols for double digestion. The erbb2 gene is inserted between XbaI and NotI. So i picked these two enzymes for digesting the...
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Is it okay stain the PVDF membrane with ponceau before adding stripping the membrane with buffers?
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How much microlitre of Phosphatase inhibitors should be added to the ripa lysis buffer ? The working range is 1:100.How much I should add for 500ul of RIPA?
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How does the peptide bonds reduce CuSO4 to cuprous ions? why cuprous ions combines with BCA and forms a complex which in turn leads to purple color formation? why protein samples are green in color?
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