few of yeast isolates isolated on YPD and PDA from metal contaminated source, is not growing on 2nd subsequent subculture. what are the possible conditions which can be considered for retrieving ?
Nilmini Kumari
Hi,
Did you change the culture media? Did you let them to grow for few days? Adequate time they may need to grow (2 ,3 days) . Addition of sugar will also enhance the growth of yeast.
Try again with a increased innoculum size.
Good luck!
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