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Questions related from Bhavya G.
There are various methods for carriyg out the SOD assay. In the paper they have mentioned the reduction of NBT is measured at 540nm. my question : a. The OD increase in the presence of SOD...
04 December 2020 8,532 0 View
they are various methods mentioned regarding the extraction of EPS. my interest is to extract loosely bond EPS from fungal culture. plz suggest me some protocols related to this. especially with...
13 June 2019 4,209 0 View
for CAT activity, is there is any threshold regarding the concentration of protein in the enzyme has to be taken into consideration before doing the experiment.
08 March 2019 3,295 5 View
experiment is to know the metal concentration in the culture broth after the incubation time through AAS. For this, should the culture broth is to be separated from the cells, then the media...
19 June 2018 8,233 4 View
As of H202 localisation studies in plant cells can we apply DAB staining for priliminary identifying of H2O2 in yeast cells under abiotic stress condition.?
11 April 2018 7,372 3 View
During the analysis of lipid peroxidization (MDA assay) as a stress bio marker under abiotic condition in microbial cells by manual method - 1. is it necessary to carry out the Standard curve ?...
11 April 2018 855 2 View
few of yeast isolates isolated on YPD and PDA from metal contaminated source, is not growing on 2nd subsequent subculture. what are the possible conditions which can be considered for retrieving ?
17 August 2017 4,659 3 View
how to calculate the molarity of 30% W/V H2O2 ? and also subsequent preparation of molar solution for enzymatic studies
03 April 2017 7,491 8 View
A plant molecule has been purified in our lab. The molecule is showing antioxidant property in blood components at 100µM after inducing stress by known oxidant (DNP), hemolysis occured as the...
08 December 2016 499 5 View
what is the concentration of H2O2 can be use in bacterial catalase enzyme study ? As per of observation few articles mention the direct use if H2O2 from 30% Stock, but few articles has carried...
09 November 2016 7,275 2 View
which method is preferred during protein extraction from bacterial source (gram positive bacteria) which will subsequently carried out for 2DE.
16 March 2016 8,587 1 View
Poacic acid is using as a anti-fungal agent in recent years. if any one worked with this, please suggest its solubility in different solvent system. thank you.
03 March 2016 4,341 2 View
The pure precipitated unknown compound obtained as the result of bacterial cellular mechanism was subjected to XRD study, result's is indicating the crystalline nature. here we are intrested to...
23 September 2015 4,266 4 View
Dear researchers please suggest a chemical methods for quantitative estimation of heavy metals like lead, cadmium, chromium and mercury except analytical methods like AOS, ICP-MS
10 July 2015 6,288 8 View
For the precipitation of DNA during extraction protocol, which is preferred, among isopropanol and isoamylalcohol and what is the underlying reason to select it ?
01 January 1970 5,279 3 View
During the in-gel activity staining of SOD, Riboflavin is used in the concentration of 28-30micromolar. As per the literature/protocols, the preparation of the riboflavin solution is in 50mM...
01 January 1970 8,456 2 View
Most of the research work related to TiO2 nanoparticle have utilized TiO2 instead of precursors. they have utilized de-ionized water as a medium. Every possible way of solubility range in acidic...
01 January 1970 4,982 4 View
I am facing a problem with the installation and opening of the px-submission tool. Can anyone give me a complete step-by-step process to run in Windows 10? Java is a recent version. I tried with...
01 January 1970 3,644 1 View