Nilmini Kumari

11 Questions 25 Answers 0 Followers

Questions related from Nilmini Kumari

Can free Karyotype softwares be used to analyse chromosome abnormalities for diagnostic purposes?

Please suggest if any free software versions are available to detect chromosome abnormalities. And if there any online courses to learn more about Karyotypying please comment. Thanks in advance

08 July 2022 642 0 View

How many restriction sites we could anchored to a primer?

I'm interesting to know that, 1. how many additional nucleotides can you add at the 5' site of the primer ? 2. How many restriction sequences can you add at the 5' site of the primer ? 3. when...

13 June 2018 940 5 View

Can RNA contaminate/ interfere plasmid sequencing?

When preparing plasmid samples we do RNAse treatment to remove RNA from plasmid DNA. I would like to know; is it necessary for plasmid sequencing from sanger di deoxy method ? How RNA interfere...

27 July 2017 7,279 6 View

Can copper sulfate, lead nitrate solutions sterilized by autoclave?

most of the time heavy metal ion solutions are sterilized by membrane filtration. Is there any possibility of changing oxidation state if they sterilized by autoclave due to high pressure or...

02 February 2017 5,205 5 View

If the protein contain a his tag, for western blot what sort of Ab is preferred?

My cloned gene contain His-C terminal tag. I would like to check my protein using western blot. So could you please advice me what sort of antibody could be use. I prefered to do the detection...

10 May 2016 272 3 View

In colony pcr, why some clones produce less intense bands while some are having very Intense bands in gel electrophoresis?

I use PCR to confirm the insert in my clones. Normally its getting positive or negative bands. But this time I got bands less intense than the positive with primer dimers. some clones only got...

01 April 2016 5,421 16 View

Are there any alternative start codon rather than ATG?

I have a bacterial gene sequence code for a protein but it's start position/ codon is TTG. Is it a mutation or consider as a alternative start codon?. I need to express this protein in a BL 21...

26 February 2016 6,684 6 View

Why do I get different blue colour colonies in my blue white screening?

In blue white screening of recombinant and non recombinants using IPTG-Xgal I got colour series of blue colonies. Some are very dark and some are pale in color intensity. And I can agree for the...

06 January 2016 8,099 6 View

Can operon contain more than one promoter?

In bacteria, protein coding genes are organized in an operon. If the operon contain three protein coding regions, My questions are; 1. Do all genes operate from one promoter? 2. Can individual...

16 November 2015 5,594 1 View

What are the usages of N-terminal and C- terminal fusion tags?

pET-28a(+) vector contain few fusion tags. N-terminal His.Tag and N-terminal T7.Tag along with C-terminal His.Tag. What are the individual usage of each tags? If all tags were there with my insert...

26 October 2015 8,593 10 View

How can I differentiate large size plasmids from genomic DNA ?

I have a wild type bacteria culture and want to check the presence of plasmids in it. I followed the normal plasmid isolation procedure and got a small pellet. In gel electrophoresis a lower...

26 December 2014 8,780 8 View