15 Questions 39 Answers 0 Followers
Questions related from Nilmini Kumari
Please suggest if any free software versions are available to detect chromosome abnormalities. And if there any online courses to learn more about Karyotypying please comment. Thanks in advance
08 July 2022 756 0 View
I'm interesting to know that, 1. how many additional nucleotides can you add at the 5' site of the primer ? 2. How many restriction sequences can you add at the 5' site of the primer ? 3. when...
13 June 2018 1,069 5 View
When preparing plasmid samples we do RNAse treatment to remove RNA from plasmid DNA. I would like to know; is it necessary for plasmid sequencing from sanger di deoxy method ? How RNA interfere...
27 July 2017 7,345 6 View
High concentration lead acetate (100 ppm) produce visible white color particle when added into LB broth media. What are the possible reasons for this...It could be visible even at 10 ppm level. Is...
18 May 2017 3,237 5 View
most of the time heavy metal ion solutions are sterilized by membrane filtration. Is there any possibility of changing oxidation state if they sterilized by autoclave due to high pressure or...
02 February 2017 5,267 5 View
My cloned gene contain His-C terminal tag. I would like to check my protein using western blot. So could you please advice me what sort of antibody could be use. I prefered to do the detection...
10 May 2016 320 3 View
I use PCR to confirm the insert in my clones. Normally its getting positive or negative bands. But this time I got bands less intense than the positive with primer dimers. some clones only got...
01 April 2016 5,509 16 View
I have a bacterial gene sequence code for a protein but it's start position/ codon is TTG. Is it a mutation or consider as a alternative start codon?. I need to express this protein in a BL 21...
26 February 2016 6,750 6 View
In blue white screening of recombinant and non recombinants using IPTG-Xgal I got colour series of blue colonies. Some are very dark and some are pale in color intensity. And I can agree for the...
06 January 2016 8,163 6 View
In bacteria, protein coding genes are organized in an operon. If the operon contain three protein coding regions, My questions are; 1. Do all genes operate from one promoter? 2. Can individual...
16 November 2015 5,655 1 View
pET-28a(+) vector contain few fusion tags. N-terminal His.Tag and N-terminal T7.Tag along with C-terminal His.Tag. What are the individual usage of each tags? If all tags were there with my insert...
26 October 2015 8,657 10 View
I need to express a gene which code for a inner membrane protein in a transgenic bacteria and I also do not know the promoter of the bacteria. I am going to use a expression vector with a...
24 September 2015 6,819 2 View
Can you brief a method for pre-preparation of bacteria pellets for TEM analysis. And if I use isolated membrane vesicles for the same observation, can I apply the same method ? And is this cell...
18 September 2015 6,963 1 View
I have a wild type bacteria culture and want to check the presence of plasmids in it. I followed the normal plasmid isolation procedure and got a small pellet. In gel electrophoresis a lower...
26 December 2014 8,847 8 View
I have a bacterial culture which was grown in a metal containing media. It was observed that, with the time a black color compound was appeared in the media. I want to confirm that whether this...
16 July 2014 807 9 View
