How does SEA software normally number subsystems?

Dear colleagues, What is the common subsystem numbering system in SEA modelling software (i.e. VA One, AutoSEA2)? Cheers, Feng

27 February 2021 7,073 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How to remove the species data from rda data frame in R (only remain the sites/samples point)?

Dear everyone, When I using the rda function in r, I met this question. As you can see in the figure, there are a lot of deep blue points which refers to the species. But I would like remain...

15 February 2021 8,982 2 View

How to Extract DNA from dried rhizomes, wood, galls etc. stored for a long time?

Dear Respectable Scientists, I have been trying to extract DNA from above mentioned sources using different CTAB/SDS Buffer Compositions and purifying agents but I am not able to extract DNA from...

12 January 2021 5,899 8 View

What are state-of-the-art methods/techniques/algorithms for the root-cause analysis of the anomalies?

I have been looking for research articles that target the question "why did an anomaly occur?", however, most of the papers that I found are related to "if an anomaly occurred?". I searched Google...

12 January 2021 7,951 3 View

Cross country comparison with matched pairs design?

Hi, I'm doing a cross-country comparison. My data for each country has a match pair design i.e. each company within a country is match with a control firm from that country. The data is...

07 January 2021 4,983 6 View

Find value of heat transfer coefficient in SI units??

A tube is subjected to air at standard temperature and pressure conditions with velocity 5 m/s. initial temperature of the tube is 980 K and drops to 500 K after interaction with the cool air....

21 December 2020 6,408 3 View

Stably transfected A549 cells are getting washed off during a plate based assay?

HI, I have transfected A549 cells with a plasmid of interest. The transfectants have been stably selected and clonally separated. I am doing a plate based fluorescence assay with the transfectants...

20 December 2020 1,222 4 View

Lyophilization problem related to Bacteria derived Gold Nanoparticles?

Respected Dr Yuri and Rawle We have prepared the bacteria derived gold nanoparticles and for its antibacterial and antioxidant applications we want exact concentration and for that purpose we...

25 November 2020 2,565 3 View

What are the data analysis methods/techniques that can be used for matched pair data?

I have data in matched pair with one experimental group and one control group. I used conditional logistic regression for my analysis. Is there any other method which can be employed for data...

14 November 2020 9,034 2 View

Do you know of an informal caregiver of a person who has recently received a bone marrow transplant?

We are conducting a pilot study of an intervention for informal caregivers of persons who have recently received a bone marrow transplant in the US. If you know of anyone who might be interested,...

13 February 2021 9,174 1 View

How to model Type 1 collagen structure for finite element analysis ?

I have started a project in which stress distribution in Type 1-collagen scaffold is to be modelled. I am thinking of using finite element analysis to model the structure and the loading...

07 February 2021 5,487 3 View

What is the difference between human umbilical vein endothelial cells (HUVECs) and bone marrow endothelial cells?

Hello I am new to work on endothelial cells, and I have been reading about them a lot and trying to know the difference between human umbilical vein endothelial cells (HUVECs) and bone marrow...

28 January 2021 8,765 4 View

How to gate mouse MDSC cells through flow cytometry?

On inducing the mouse bone marrow cells with X compound, there is a change in cells morphology and characteristics. In that case how to gate these cells, as these cells are showing suppressive...

27 January 2021 608 2 View

How much of cells needed to get good number of RNA ?

Hello I am growing MSCs cells in either 24 wells or 12 wells plate and then I need to lysate these cells for RNA extraction I ususally get RNA from 5 to 30s ng/ul Is there is any idea or tips...

26 January 2021 8,531 3 View

How to do rat bone Immunofluorescence, Please suggest?

Hello, I have been trying to do a rat bone Immunofluorescence histology study. But not able to find a successful protocol till now, Any suggestions? Rat bone: Decalcifies Embedding: Cryo

26 January 2021 4,165 3 View

Hello, my name is siti. My question is what is the related issue in bone marrow transplant or hematology that I can choose for my research project ?

I am doing a master in nursing but still does not have any idea about what topic I am going to choose. pls help

25 January 2021 1,097 12 View

I am culturing murine bone marrow derived mesenchymal stromal cells, what are these cells I am observing?

Dear all, I am isolating mesenchymal stromal cells (MSCs) from the bone marrow of adult C57BL/6 mice. The culture conditions are: -IMDM + L-glut, 20% of 50:50 FBS:HS, 1% P/S -Medium replaced each...

24 January 2021 7,223 3 View

How to rotate an anisotropic stiffness matrix to an orthotropic matrix of cancellous bone?

Hi everyone, recently i have a question about how to calculate the orthotropic elastic matrix of cancellous bone cube. I really need the help!!!! I applied six stress states to the cancellous...

21 January 2021 9,143 2 View

How to create a scaffold by using the particle leaching process?

In the leaching process,Why does NaCl settle despite sieving? How can we understand how much solvent the system needs? And, how can enhance the mechanical strength in this process and find the...

12 January 2021 5,679 2 View