Question on detection of DNA Protein covalent complexes by dot blot. How to achieve reproducubility?

I am trying to detect DNA-protein covalent crosslinks which are induced by my drug treatment. I am following the RADAR assay protocol. Briefly, I am lysing cells using a buffer containing 6M GITC,...

30 July 2020 949 4 View

What is special about Marketplace Simulations that make them ideal for online classes?

07 June 2020 3,212 9 View

You'r Opinion on Understanding the Voice of the Customer (VOC) through AI?

VOC is certainly nothing new. The more brands understand their customers, the better they are able to create products and services that meet their needs, gain competitive intelligence, and...

25 May 2020 6,220 6 View

Do you think privacy issues in social media is a major concern for consumers?

As we all know we share lot of information in social media, which is accessible by anybody around the world. Do you really think this is a threat to the consumers? Do consumers try to protect...

18 May 2020 3,900 18 View

Is it possible for Co simulations with IES-VE.?

Can I couple IES-VE with other tools like MATLab or Modelica? My research is focused on control for better thermal comfort and indoor air quality. So I want to use MATlab or Modelica, it will be...

28 April 2020 6,540 5 View

I need ANOVA software any one having send to the link or send to my mail id [email protected]?

i need optimization for wear tests using the Taguchi method.

30 December 2019 5,446 2 View

Is staurosporine can use as a positive control for caspase staining on frozen sections?

How can we use staurosporine as a positive control for caspase staining on frozen sections

23 May 2019 9,780 2 View

How to do molecular dynamic simulation?

I want to carryout molecular dynamic simulation of some amphiphilic molecules to study their assembling behavior. My particular interest is in the lamellar bilayer arrangements. Someone, please...

16 May 2019 9,997 7 View

Do states, inputs, and outputs used in observer have to be the same as states, inputs, and outputs used in controller?

I'm designing an extended Kalman filter observer in a control system. In the control system, there is an MPC controller. Do states, inputs, and outputs used in observer have to be the same as...

17 March 2019 4,114 3 View

How can we reduce the autofluorescence or background fluorescence in immunohistochemistry ?

Can anyone give suggestions for reducing the autofluorescence/background fluorescence of whole eye cryosctions in immunohistochemistry. The area of interest is retinal photoreceptor layer.

19 July 2018 5,555 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View