Hi,

I am planning to test a drug that is known to reduce phosphorylation of RNA poll II at Ser2.

My question is when I collect lysate for western do I need to do nuclear fractionation or is whole cell lysate sufficient?

This is because I read that chromatin bound proteins might be hard to detect from whole cell lysates but I do see westerns of RNA pol II from whole cell lysate on images of representative western blots on the antibody webpages online.

Thanks