Could you suggest from where can I find the EEG data set of patients with Disorder of Consciousness?

I am a research scholar working in brain computer interface for DOC patients. I am looking for EEG data set specifically of the patients with consciousness disorder. Could you suggest any references?

25 August 2020 3,969 2 View

What is special about Marketplace Simulations that make them ideal for online classes?

07 June 2020 3,212 9 View

You'r Opinion on Understanding the Voice of the Customer (VOC) through AI?

VOC is certainly nothing new. The more brands understand their customers, the better they are able to create products and services that meet their needs, gain competitive intelligence, and...

25 May 2020 6,220 6 View

Do you think privacy issues in social media is a major concern for consumers?

As we all know we share lot of information in social media, which is accessible by anybody around the world. Do you really think this is a threat to the consumers? Do consumers try to protect...

18 May 2020 3,900 18 View

Is it possible for Co simulations with IES-VE.?

Can I couple IES-VE with other tools like MATLab or Modelica? My research is focused on control for better thermal comfort and indoor air quality. So I want to use MATlab or Modelica, it will be...

28 April 2020 6,540 5 View

I need ANOVA software any one having send to the link or send to my mail id [email protected]?

i need optimization for wear tests using the Taguchi method.

30 December 2019 5,446 2 View

How to solve the high distortion error in two way FSI simulations?

I am trying to simulate the blood flow in arteries by two way FSI (Fluent and Transient Structural). Every time I get the error of this sort: "(DP 0) Element 191 located in Body "Solid" (and...

12 December 2019 8,400 2 View

Is staurosporine can use as a positive control for caspase staining on frozen sections?

How can we use staurosporine as a positive control for caspase staining on frozen sections

23 May 2019 9,780 2 View

How can we reduce the autofluorescence or background fluorescence in immunohistochemistry ?

Can anyone give suggestions for reducing the autofluorescence/background fluorescence of whole eye cryosctions in immunohistochemistry. The area of interest is retinal photoreceptor layer.

19 July 2018 5,555 5 View

Why Zinc oxide is naturally n type and nickel oxide is naturally p type semiconductors ?

why ZnO acquire n type behavior regardless the fabrication method similar to the p type behavior of NiO nano particles. I like to know about the physics behind it.

29 December 2017 539 14 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View