Hi:
Immunotherapy / Allograft questions
I have question about how many cells should I inject to BALB/C mouse ? I have had my own research for the amount of cell injection to mouse, and didn't use matrigel when cell injection. Some research articles didn't use matrigel, some did use one. and some articles have different opinions with other articles.
Well, the CT26 I injected to BALB/C mouse grows well and steady.
However, 5,000,000 cells (this is what I injected) can be too many (I guess) which is almost 10x than usual amount.
So, these are the questions
1) how many cells you guys injected in allograft model.
2) do you guys use metrigel ?
3) if you guys use matrigel, is the number of cells changed ? if so, how many cells do you guys inject when you use matrigel ?
Thank you !
CT26 grows double in 16hours fast.
Thanks