depends from what tumor model you need to study, if it is a tumor from Balb or B6 background. anyway, if you can use different mouse models and different tumors in your experiments it will add value and significance to your research.

It depends on 3 factors:

1-Tumor cell-line

2-Type of immunotherapy approach

3-Cancer type

For example, the most commonly used tumor model for melanoma is the B16 and the MC38 colon cancer model were generated in the C57/B6 strain, while a sophisticated breast cancer model like the 4T1 and the colon cancer CT26 were generated in BALB/c.

It also depends on what cell type you want to study; Some tumors favor MDSC and myeloid infiltration and therefore are labeled as "cold tumor" where T-cell recruitment is low...While other tumors favor immunogenic responses and significant T cell recruitment so are "hot tumors"...Whether your lab focuses on T cells or myeloid cells or both, your tumor type and by consequence, mouse strain would be important.

The immunotherapeutic approach that you will use will also be relevant. For example, BALB/c mice favor better humoral responses as well Th2 immunity while B6 mice have a stronger Th1 response.

The microbiome is now appreciated to have a predominant role in tumor responses and as expected, there are substantial differences in the microbiome of B6 vs BALB/c mice.

It all depends on what you plan to study in the context of immunotherapy.

Thanks for the answer ! I am gonna use CT26wt or MC38 and for the rechallenge experiment, I will be using 4T1.

It depends on 3 factors:

1-Tumor cell-line

2-Type of immunotherapy approach

3-Cancer type

For example, the most commonly used tumor model for melanoma is the B16 and the MC38 colon cancer model were generated in the C57/B6 strain, while a sophisticated breast cancer model like the 4T1 and the colon cancer CT26 were generated in BALB/c.

It also depends on what cell type you want to study; Some tumors favor MDSC and myeloid infiltration and therefore are labeled as "cold tumor" where T-cell recruitment is low...While other tumors favor immunogenic responses and significant T cell recruitment so are "hot tumors"...Whether your lab focuses on T cells or myeloid cells or both, your tumor type and by consequence, mouse strain would be important.

The immunotherapeutic approach that you will use will also be relevant. For example, BALB/c mice favor better humoral responses as well Th2 immunity while B6 mice have a stronger Th1 response.

The microbiome is now appreciated to have a predominant role in tumor responses and as expected, there are substantial differences in the microbiome of B6 vs BALB/c mice.

It all depends on what you plan to study in the context of immunotherapy.

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Thanks for the answer

I have CT26 and MC38, and I am going to do the treatment research.

so the thing is that I am going to inject those cell and do the combination treatment and see how the tumor size is decreased...

I am going to use PD-L1 antibody though. so the more question is that if I use PD-L1 antibody, it seems like it is better to use BALB/c rather than C57 because of BALB/c has better effect with humoral response right ?

I have one more question about the re-challenge experiments.

some people use B16 which grows like a monster from mars.. it sometimes scares me that it grows so fast..

people use B16, how can they do the re-challenge experiment ? can it be controlled?

thank you !