How many B16F10 cells should I inject per mouse ?

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Why do we use HCl salt with the drug???

I have silly question about why putting HCl salts with the drug. For example, I have doxorubicin and it says DOX with hcl salt. wel, I heard that this thing makes DOX better solubility....

31 December 2018 1,523 0 View

Question about thermodynamics ? Hydrogen bonding breaking ?

I am having trouble with this calculation, well, this must be so easy for you guys who are majoring in this field though. (since it is not calculate question) Well, I am having difficulty to...

10 November 2018 1,400 0 View

Questions about number of cell injection to mouse in Immunotherapy ?

Hi: Immunotherapy / Allograft questions I have question about how many cells should I inject to BALB/C mouse ? I have had my own research for the amount of cell injection to mouse, and didn't...

10 November 2018 6,107 0 View

Question about flow cytometry for immunotherapy ?

Hi: I have a emergence question about the FACs in immunotherapy.. Any comment will be helpful.. I am going to go immunotherapy and this is my first project so I am having hard time to figure...

09 October 2018 3,667 3 View

Question about immuno antibody (PD-L1) & atezolizumab ?

Hi: I have question on the PD-L1 antibody. I am using BALB/C mouse for immunotherapy and I am using Atezolizumab. I searched internet that it is ok to use human IgG antibody to mouse which I...

08 September 2018 9,272 0 View

Does antibody have thiol group (-SH) on the outside surface so that it can conjugate with maleimide ?

Hi: Antibody has -S- at the center which is very common to everyone. But I am going to try to conjugate with other thiol groups (if there is one). Did anybody try conjugation between -SH &...

08 September 2018 1,684 0 View

How many thiol groups in a antibody (IgG)?

Hi Any body know how many thiol groups in a antibody ? (IgG) and where they are located ? thx !

08 September 2018 9,256 0 View

BALB/c ? C56BL/6 ? which one is better mouse model for immunotherapy?

I am wondering if there is any big difference btwn those two mice models. beside the fact that C56 is more aggressive, I have no idea which one is better for immunotherapy. anyone can help me

07 August 2018 7,776 4 View

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

How to calculate battery consumption for IoT device?

Hi, everyone I'm working on a privacy-preserving algorithm that will apply to IoT devices. So, I need to measure the power consumption by implementing this algorithm. Any suggestion, advice,...

25 February 2021 8,749 6 View

How vulnerable is water and wastewater infrastructure to cyber terrorism in an age of IoT?

Im looking for any papers, reports,articles, links etc on information on How vulnerable is water and wastewater infrastructure to cyber terrorism in an age of IoT.

24 February 2021 2,750 4 View

Help with culturing monolayer of human brain microvascular endothelial cells (hBMEC)?

I've been having a hard time forming an endothelial monolayer with good tight junctions. The person who trained me on the culture of endothelial cells worked mostly with mouse BMEC, and he used...

22 February 2021 8,253 1 View

Open Source IoT Simulators supported by Machine Learning?

Dear Research Gaters, Let me first add context to the question. I am looking to simulate the waste management system and resources of a city. The hardware devices I need to simulate include,...

21 February 2021 2,333 4 View