Does antibody have thiol group (-SH) on the outside surface so that it can conjugate with maleimide ?

09 September 2018 0 2K Report

Hi:

Antibody has -S- at the center which is very common to everyone. But I am going to try to conjugate with other thiol groups (if there is one). Did anybody try conjugation between -SH & maleimide ?

does it work ?

thanks ~

Badges
Science topic

More Daehyun Kim's questions See All

Why do we use HCl salt with the drug???

I have silly question about why putting HCl salts with the drug. For example, I have doxorubicin and it says DOX with hcl salt. wel, I heard that this thing makes DOX better solubility....

31 December 2018 1,523 0 View

Question about thermodynamics ? Hydrogen bonding breaking ?

I am having trouble with this calculation, well, this must be so easy for you guys who are majoring in this field though. (since it is not calculate question) Well, I am having difficulty to...

10 November 2018 1,400 0 View

Questions about number of cell injection to mouse in Immunotherapy ?

Hi: Immunotherapy / Allograft questions I have question about how many cells should I inject to BALB/C mouse ? I have had my own research for the amount of cell injection to mouse, and didn't...

10 November 2018 6,107 0 View

Question about flow cytometry for immunotherapy ?

Hi: I have a emergence question about the FACs in immunotherapy.. Any comment will be helpful.. I am going to go immunotherapy and this is my first project so I am having hard time to figure...

09 October 2018 3,667 3 View

Question about immuno antibody (PD-L1) & atezolizumab ?

Hi: I have question on the PD-L1 antibody. I am using BALB/C mouse for immunotherapy and I am using Atezolizumab. I searched internet that it is ok to use human IgG antibody to mouse which I...

08 September 2018 9,272 0 View

How many thiol groups in a antibody (IgG)?

Hi Any body know how many thiol groups in a antibody ? (IgG) and where they are located ? thx !

08 September 2018 9,256 0 View

BALB/c ? C56BL/6 ? which one is better mouse model for immunotherapy?

I am wondering if there is any big difference btwn those two mice models. beside the fact that C56 is more aggressive, I have no idea which one is better for immunotherapy. anyone can help me

07 August 2018 7,776 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View