Question about flow cytometry for immunotherapy ?

10 October 2018 3 4K Report

Hi:

I have a emergence question about the FACs in immunotherapy..

Any comment will be helpful..

I am going to go immunotherapy and this is my first project so I am having hard time to figure out what data I need to get.

I have kept searching for research article and read alot as well however, I think it is going to be much easier to get ideas if you guys can point out my questions. So please direct me and give me some basic info which will be so helpful to get better ideas and get better understand while I am reading research articles and for my research understanding.

my question is,

In immunotherapy, I need to use FACs to check if there is immune response, like T cell, NK, variety cytokines and etc..

can you guys point out what I need to search from FACs?

example, T cell/NK/IFN-gamma/ and so on..

so that I get the idea that what I can or need to find data from FACs.

Also,

Can you guys point me out what I need to search from other methods for other immune response ? like ELISA, or IHC... and so on.

I think IHC is for to see the T cell on the tumor tissue but I am not 100percent sure that this is only thing I can find in IHC..

Thank you very much !

Olivier Lucar

Hello,

Can you give me more information regarding the immunotherapy project you are doing? are you taking a interested in immune checkpoints ? If you want to assess the immune function of T cells or NK cells so yes FACS is the best option. Do you have access to a FACS machine of at least 10 colors ?

Daehyun Kim

Olivier Lucar You just made me embarrassed. Why didn't I think like that ? :)

Thank you so much for your answer !!

So my last question is that yes, since there are not many affordable colors, then you mean T cells , NK cell , one more (if there are 3 colors) is the best option. then what other cytokines and other immune can be detected then? Should I do FACs multiple times then ? or is there other cool method to figure out about other specific cytokines or any other immune things ?

Your FACS has only 3 colors ? If so it is going to be difficult to asses every population and function at the same time. If you are interested for instance in NK cells you need to isolate them first (by magnetic beads for instance) and then look at cytokines expression (IFN-gamma, TNF-alpha, MIP-1B...) or cytotoxic (CD107a, granzyme B,...)

Why do we use HCl salt with the drug???

Question about thermodynamics ? Hydrogen bonding breaking ?

Questions about number of cell injection to mouse in Immunotherapy ?

Question about immuno antibody (PD-L1) & atezolizumab ?

Does antibody have thiol group (-SH) on the outside surface so that it can conjugate with maleimide ?

How many thiol groups in a antibody (IgG)?

BALB/c ? C56BL/6 ? which one is better mouse model for immunotherapy?

How to compare two groups with only two measurements?

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

What are the values needed for gabor filter's parameter for cifar10 data?

No title

What techniques can I use to determine the miscibility of polymer blends?

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Where can I find life cycle inventory or assessment of non woven materials?

Does Napabucasin soluble in dichloromethane and in ethanol?

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Help request in CST microwave?