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Hi everyone, I'd like to better separate two close peaks coming from a bioconversion sample, I'm currently using a C18 KromaPhase 4,6mm Ø.I. SCHARLAU, particle size (µm): 10, pore size (Å): 100,...
30 July 2024 435 3 View
The as the solution were mixed together, there was no color change, and then the UV-vis was read every 10min but barely any absorbance was observed. The solutions were 2,1mM of NaBH4 and 0,07g...
20 May 2024 615 1 View
Can anyone tell me the amount of NPK required per plant for sweet pepper to be grown in pot?
15 April 2024 9,388 0 View
Can anyone tell me the amount of NPK required per plant for cucumber to be grown in pot? Thanks in advance
15 April 2024 3,040 0 View
Hej everyone, I'm working with humic substances in soil amendments. Last year, I outsourced measurements for fulvic and humic acids to a lab. After months, I sent freshly made samples to a...
05 February 2024 8,964 3 View
I need to determine the concentration of organic forms of nitrogen in a fertilizer and distinguish them from urea. The fertilizer is composed by ammonium nitrate, urea, and these organic forms....
05 February 2024 5,133 6 View
Durante los últimos tres años he estudiado los procesos de inserción laboral de los ex becarios mexicanos del Programa Erasmus+ que involucra a los países de la Unión Europea. En una segunda etapa...
05 October 2023 8,097 0 View
"Breaking the Issue: Quantitative Analysis of Public Perception and Reaction to Cancel Culture Incidents on Twitter"
19 September 2023 1,314 4 View
What descriptions does the author use to show a change in scenery? What order is the information in: chronological, spatial, or importance? Does recognizing the order help you better understand...
26 August 2023 8,690 0 View
What are the differences between Spurway and CAT+ extraction of bioavailable nutrients, aside from the extraction agent (acetic acid and calcium chloride respectively)?
02 January 2023 7,994 3 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...
28 July 2024 5,658 0 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...
25 July 2024 5,001 2 View
"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...
23 July 2024 9,109 0 View
Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...
17 July 2024 2,787 4 View
Occasionally we observe lack of staining in the inner part of a skin section processed with indirect immunofluorescence. Can be due to incomplete removal of the cryoprotectan during the wash? Any...
07 July 2024 8,200 2 View
