Recombinant protein concentration for an in vitro assay?

Hello everyone, I would like to observe the effects of a recombinant protein on in vitro cultured cells. For this reason, I need to know a concentration at which recombinant proteins can be used...

28 May 2020 5,304 3 View

How long does it last the labelling with PKH26 on EVs?

Hello everyone, I have a concern about the EVs staining with the lipophylic dye PKH26. I would like to stain EVs and then inject them in order to assess their distribution within the liver. My...

12 May 2019 8,621 3 View

How to stain exosomes with PKH26?

Hello people, I would like to stain EVs and see whether, once stained, they are captured by cells or they interact with cell surface. I was looking to the manifacturer's instructions and I...

02 December 2018 6,486 2 View

Is there a method to check for RNA integrity/quality that does not require to run an agarose gel nor to use Bioanalyzers?

Hi everybody, I would like to assess the quality of my RNA before performing further analyses (RT-qPCR) but I do not have access to Bioanalyzers like the Experion or Agilent 2100 Bioanalyzer. I...

06 February 2018 927 2 View

Software for generating synthetic stream flows?

04 May 2017 4,738 1 View

Could someone tell me if Glycoblue (ThermoFisher) can affect Nanodrop readings of RNA samples?

I use Glycoblue to dye my RNA pellets deriving from cytosolic or nuclear RNA (concentrations ranging from 150 to 600 ng/uL) and everything is pretty fine with the absorbance ratios from...

03 May 2017 5,959 4 View

How can I prepare salivary simulated fluid?

23 November 2016 2,141 3 View

Which is the relationship between Collaborative Innovation and Open Innovation?

We are developing an interdisciplinary study, which relates Communication discipline and Innovation theory. For that reason, we want to know, how does this relationship work? Do you know papers...

04 May 2016 3,897 0 View

Are you familiar with path analysis with distance matrices?

I would like to perform a path analysis but I only have 4 distance matrices. First, can I really do it? And what software should I use?

12 April 2015 3,468 3 View

Are you familiar with path analysis with distance matrices?

I would like to perform a path analysis but I only have 4 distance matrices. First, can I really do it? And what software should I use?

03 April 2015 1,429 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Does the property of GFP affect a fusion protein's localization?

Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...

15 February 2021 4,353 3 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View