Recombinant protein concentration for an in vitro assay?

Hello everyone, I would like to observe the effects of a recombinant protein on in vitro cultured cells. For this reason, I need to know a concentration at which recombinant proteins can be used...

28 May 2020 5,304 3 View

How long does it last the labelling with PKH26 on EVs?

Hello everyone, I have a concern about the EVs staining with the lipophylic dye PKH26. I would like to stain EVs and then inject them in order to assess their distribution within the liver. My...

12 May 2019 8,621 3 View

How to stain exosomes with PKH26?

Hello people, I would like to stain EVs and see whether, once stained, they are captured by cells or they interact with cell surface. I was looking to the manifacturer's instructions and I...

02 December 2018 6,486 2 View

Is there a method to check for RNA integrity/quality that does not require to run an agarose gel nor to use Bioanalyzers?

Hi everybody, I would like to assess the quality of my RNA before performing further analyses (RT-qPCR) but I do not have access to Bioanalyzers like the Experion or Agilent 2100 Bioanalyzer. I...

06 February 2018 927 2 View

Software for generating synthetic stream flows?

04 May 2017 4,738 1 View

Could someone tell me if Glycoblue (ThermoFisher) can affect Nanodrop readings of RNA samples?

I use Glycoblue to dye my RNA pellets deriving from cytosolic or nuclear RNA (concentrations ranging from 150 to 600 ng/uL) and everything is pretty fine with the absorbance ratios from...

03 May 2017 5,959 4 View

How can I prepare salivary simulated fluid?

23 November 2016 2,141 3 View

Which is the relationship between Collaborative Innovation and Open Innovation?

We are developing an interdisciplinary study, which relates Communication discipline and Innovation theory. For that reason, we want to know, how does this relationship work? Do you know papers...

04 May 2016 3,897 0 View

Are you familiar with path analysis with distance matrices?

I would like to perform a path analysis but I only have 4 distance matrices. First, can I really do it? And what software should I use?

12 April 2015 3,468 3 View

Are you familiar with path analysis with distance matrices?

I would like to perform a path analysis but I only have 4 distance matrices. First, can I really do it? And what software should I use?

03 April 2015 1,429 3 View

Does the genus Capsicum contain solanine in their stem like other nightshades do?

Hello, this is yet another urgent question for my assignment: Does the genus Capsicum contain solanine in their stem like other nightshades do? Thank you for your answers in advance!

03 June 2020 3,827 11 View

Fusarium spp. that causes wilt on Hot pepper is the same with that of Tomato and other plant?

The fungus Fusarium oxysporum that causes Fusarium wilt on Hot pepper is the same with that of Tomato plant that causes wilt on Tomato ?

06 July 2019 6,023 14 View

How much ethanol do I need in extracting capsaicin from red bell peppers?

The capsaicin recovered will be tested against aphids.

04 February 2019 5,811 5 View

How much ethanol do I need in extracting capsaicin from red bell peppers?

The capsaicin recovered will be tested against aphids.

01 February 2019 9,877 6 View

What specific locations should i get pepper sauce samples to get a representative sample for the Accra Metropolitan, if i can only do 60 samples?

My study involves testing 60 samples of red pepper sauce eaten as condiments, obtained from street food vendors. The location under study is the Accra Metropolitan. The samples will be obtained...

31 December 2018 1,988 3 View

Standard addition method for every sample?

Hi everyone! I'm trying to analyze vitamin C in several species of capsicum. Beside using different extraction methods and considering all the cares needed to protect Vit C from degradation I get...

04 May 2018 1,158 5 View

How important is the diversity of colors in the Capsicum?

The oleoresins where they are applied and what is the best solvent? acetone?

02 March 2018 5,813 0 View

What is the greatest impact / use of Capsicum in the industry / trade?

What are the applications in the food, pharmaceutical, chemical industry?

02 March 2018 2,352 1 View

What is the problem with shoot elongation in Agrobacterium-mediated Transformation ?

we are working on Agrobacterium-mediated Transformation in Capsicum annuum,after 3-4 subcultures small shoot buds are initiated but it's not elongated next....

05 June 2016 7,733 5 View

Has anyone done a genetic transformation of tomato incorporating the gene for capsaicin from hot pepper (Capsicum annuum or C. chinense)?

14 May 2015 5,991 7 View