Recombinant protein concentration for an in vitro assay?

Hello everyone, I would like to observe the effects of a recombinant protein on in vitro cultured cells. For this reason, I need to know a concentration at which recombinant proteins can be used...

28 May 2020 5,304 3 View

How long does it last the labelling with PKH26 on EVs?

Hello everyone, I have a concern about the EVs staining with the lipophylic dye PKH26. I would like to stain EVs and then inject them in order to assess their distribution within the liver. My...

12 May 2019 8,621 3 View

How to stain exosomes with PKH26?

Hello people, I would like to stain EVs and see whether, once stained, they are captured by cells or they interact with cell surface. I was looking to the manifacturer's instructions and I...

02 December 2018 6,486 2 View

Is there a method to check for RNA integrity/quality that does not require to run an agarose gel nor to use Bioanalyzers?

Hi everybody, I would like to assess the quality of my RNA before performing further analyses (RT-qPCR) but I do not have access to Bioanalyzers like the Experion or Agilent 2100 Bioanalyzer. I...

06 February 2018 927 2 View

Software for generating synthetic stream flows?

04 May 2017 4,738 1 View

Could someone tell me if Glycoblue (ThermoFisher) can affect Nanodrop readings of RNA samples?

I use Glycoblue to dye my RNA pellets deriving from cytosolic or nuclear RNA (concentrations ranging from 150 to 600 ng/uL) and everything is pretty fine with the absorbance ratios from...

03 May 2017 5,959 4 View

How can I prepare salivary simulated fluid?

23 November 2016 2,141 3 View

Which is the relationship between Collaborative Innovation and Open Innovation?

We are developing an interdisciplinary study, which relates Communication discipline and Innovation theory. For that reason, we want to know, how does this relationship work? Do you know papers...

04 May 2016 3,897 0 View

Are you familiar with path analysis with distance matrices?

I would like to perform a path analysis but I only have 4 distance matrices. First, can I really do it? And what software should I use?

12 April 2015 3,468 3 View

Are you familiar with path analysis with distance matrices?

I would like to perform a path analysis but I only have 4 distance matrices. First, can I really do it? And what software should I use?

03 April 2015 1,429 3 View

METHOD: What method to use for transient gene silencing in Cucumis sativus ?

I'm planning to silence candidate genes in the root system of C. sativus and viral vectors seem scarce in Cucurbitaceae and with low efficacy. Therefore, I'm looking for people who have experience...

06 December 2020 1,716 1 View

Someone from Alexandria University his name Mohammed Salem claimed three of my papers to him in research gate, how can this problem be resolved?

Mohammad Salem from the Alexandria University claimed the authorship these three papers: 1. First Report of Fusarium verticilloides causing Banana Fruit Rot in Jordan 2. First report of cucurbit...

26 July 2020 9,647 3 View

How to calculate PAL activity in cucumber leaves?

Hi everyone I am doing PAL assay in cucumber leaves and I am using the paper below as a reference I don't understand how they convert the absorbance value to enzymatic units They indicate that...

31 December 2019 4,361 3 View

Can someone name root-knot resistant cucumber cultivars?

Cultivars to resistant ot root-knot nematodes

01 June 2019 9,969 5 View

Whats the most reliable way to calculate leaf area (Cucumber) without using any instrument?

with simple scale measuring its length and width and addition of correction factor is reliable or not?.

10 November 2018 539 6 View

Best staining methods to ensure the effective AMF inoculation?

I am working on Arbuscular Mycorrhizal Fungi (AMF) with emphasizing on Cucumber productivity in the presence or co-inoculation with organic substrate, Could someone guide how to check the...

04 May 2018 8,787 9 View

Cucumber plants have short internodes and curly leaves, cracked fruits, what is the reason?

There is a strong deformation of plant tops, leaves, fruits crack along and filled with smelly liquid. The cross-and longitudinal sections free of any symptoms of systemic infection. More than...

02 March 2018 4,970 2 View

How can I use my polyhouse in Summer season in hot n humid climate of Odisha, India where temperature go up 45 degree c???

I hv a polyhouse in experimental field. I hv experimented parthenocarpic cucumber inside it during winter n got bumper production. But now in march the temperature in my area touches 49 degree n...

02 March 2018 1,966 5 View

Is there any method to find the cluster initial in K-means clustering other than randomly?

I am working in a project with identifying cucumber fruits in a green house environment. i have followed some research articles and found K-means clustering for segmentation process. defining the...

11 December 2017 844 3 View

What is the reason for this infection?

cucumber

06 July 2017 5,424 4 View