When making the SDS-PAGE running and stacking gel solution, can I save the solution without adding the TEMED and APS for future experiments? If so, how long can I save it?
Youngjoo Jo you can combine the SDS and Tris buffers and leave at room temperature as normal. However, acrylamide needs to be kept at 4 oC for long term storage. If you are in a hurry, I would set up a few tubes containing the Tris buffers and SDS which can be stored for up to a year and simply add acrylamide, APS and TEMED as you need to.
For how long? You can combine Tris, water and SDS and leave them in RT (SDS precipitates at 4°C, but if heated a little bit it becomes soluble again), and add TEMED, APS, and Acrylamide just before using them.
Yes you can save the running and stacking gel buffers at 4°C without adding the TEMED and APS for future experiment. Only thing during storage, it should not be turbid and the pH should be same.
Their pH should should be maintained, any change in the pH of respective buffers would effect the result The difference in pH of stacking gel(6.8) and resolving gel(8.8) plays a major role in separation of peptides according to their molecular weight.
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