Youngjoo Jo

5 Questions 2 Answers 0 Followers

Questions related from Youngjoo Jo

When DTNB react with free thiol, how can I remove it to obtain thiol-free sample?

I'm working on synthesizing formyl-CoA. If I add DTNB into mixed sample, it forms mixed disulfide and TNB2- which is yellow color. How can I remove these two compounds from my mixed sample and can...

29 June 2021 9,562 0 View

Formyl-CoA detection?

I have a freshly synthesized formyl-CoA but not sure if it really is. Some published articles introduce that they used ToF Mass spectroscopy. But as a matter of money and time, for me it will be...

24 June 2021 9,006 1 View

Aminex HPX-87H column washing for 16 hours?

Hi! I just started using a brand new HPX-87H column to analyze organic acids using HPLC. I've only used C18 columns before and the washing process took only 90min. But now this HPX-87H, according...

06 June 2021 2,925 3 View

Difference between DB-5ms and HP-5ms column in GC-MS?

I'm planning to identify the compound which is expected to be a long chain carbon aldehyde(~C16), and for now we have DB-5ms column. I checked the chemical structural difference between the two,...

23 March 2021 7,827 7 View

Question about the SDS-PAGE gel solution preparation?

When making the SDS-PAGE running and stacking gel solution, can I save the solution without adding the TEMED and APS for future experiments? If so, how long can I save it?

29 January 2021 3,948 3 View