I did this SDS PAGE GEL and my results are not precise or improving.
can someone guide me through the trouble?
Hi Hasnat,
You are overloading your gels, that is why the protein profile of the samples you have loaded look like this. Load less amount of extract (I would say 1/3). Also, the horizontal lines you are observing across the gel, if I am not wrong, comes from contamination of your reagents to prepare gels. If any buffer/acrylamide is contaminated with keratin from our skin or just protein contamination from mishandling, it will appear like this in your gel. I would make new stock reagents to prepare gels.
What is the size of the protein of your interest? Prepare your gels with appropriate acrylamide concentration to see your protein properly. I don't know if your protein of interest is the band that is at the bottom of the gel, then raise the acrylamide concentration so smaller proteins are better resolved.
- Badges
- Science method