Xi Jiang

3 Questions 67 Answers 0 Followers

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Can amino acid change after protease cleavage?

Hello everyone, I am currently working with targeting proteins to yeast mitochondria. Our strategy is to use a mitochondrial targeting signal to transport our protein of interest to mitochondrial...

17 October 2019 6,682 5 View

Why is my his-tagged protein not detected in my western blots?

Dear all, I am having problems with my Western blots. I am doing transient expression of proteins in Nicotiana benthamiana using Agrobacterium tumefaciens. These two proteins are both his-tagged...

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Has someone any tips for getting short (<100bp) PCR products ?

Hi guys, I am starting with a small project for my dissertation. And the first problem I have is that I am stuck with a cloning because of my bad PCR products yield. I am having many troubles to...

20 November 2015 7,806 2 View