protocol for lysis buffer and TE buffer for the extraction of DNA from insects .?

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Hello everyboy, i need help How to do Rapid amplification of cDNA ends (RACE)?

Hello everyboy, i need help How to do Rapid amplification of cDNA ends (RACE)? i have got the sequence of two new genes in my species (Insect) now i want to do Rapid amplification of cDNA ends...

06 July 2018 8,932 2 View

Any suggestion on why different predicted band sizes appear for the same genes but different spp...?

Any suggestion on why different predicted band size appear for the same gene but different spp...? i am using primers for simple PCR amplification from previously publish paper. In published paper...

04 May 2018 6,989 6 View

How to make a lysis buffer for the extraction DNA from insects?

hello @ all researchers, i want to extract DNA from the midgut of insect so i need to know how to make a lysis buffer..? thanks

02 March 2018 4,975 3 View

In vitro metabolism of chlorantraniliprole insecticide..?

hello everyone, i am working on detoxification enzymes activities in insects. i need the protocol of in vitro metabolism of chlorantraniliprole insecticide.. Thanks

02 March 2018 4,035 0 View

Greeting,

I need this paper, Stuties on insecticide susceptibility of the beet armyworm fed on different host plants

06 July 2017 3,577 0 View

Hello, How do make a standard curve of alpha naphthol for estimating esterase enzyme activity? .

Please suggest how to dissolve the naphthol in buffer for making standard curve

06 July 2017 7,376 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View