Hello everyboy, i need help How to do Rapid amplification of cDNA ends (RACE)?

Hello everyboy, i need help How to do Rapid amplification of cDNA ends (RACE)? i have got the sequence of two new genes in my species (Insect) now i want to do Rapid amplification of cDNA ends...

06 July 2018 8,762 2 View

Any suggestion on why different predicted band sizes appear for the same genes but different spp...?

Any suggestion on why different predicted band size appear for the same gene but different spp...? i am using primers for simple PCR amplification from previously publish paper. In published paper...

04 May 2018 6,868 6 View

How to make a lysis buffer for the extraction DNA from insects?

hello @ all researchers, i want to extract DNA from the midgut of insect so i need to know how to make a lysis buffer..? thanks

02 March 2018 4,870 3 View

protocol for lysis buffer and TE buffer for the extraction of DNA from insects .?

I need the protocol for lysis buffer and TE buffer for the extraction of DNA from insects Thanks

02 March 2018 994 4 View

In vitro metabolism of chlorantraniliprole insecticide..?

hello everyone, i am working on detoxification enzymes activities in insects. i need the protocol of in vitro metabolism of chlorantraniliprole insecticide.. Thanks

02 March 2018 3,904 0 View

Hello, How do make a standard curve of alpha naphthol for estimating esterase enzyme activity? .

Please suggest how to dissolve the naphthol in buffer for making standard curve

06 July 2017 7,184 2 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

What is this fern?

Please help me to identify this fern species. Thank you in advance.

24 February 2021 2,435 5 View

Please help in identification of this insect?

This insect is minute in size around 1 mm and not clearly visible with naked eye.

22 February 2021 5,907 11 View

How to use Remote sensing and GIS applications for mapping the available medicinal plants within the locality/municipality?

Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...

21 February 2021 374 5 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Can medicinal plants really play an inherent rule in the COVID-19 tackle?

Numerous scientific reports cited plant extracts with potential antiviral activity and even anti-COVID-19? Why these findings are neglected? I remember very well the warning released by the WHO to...

17 February 2021 2,141 9 View

Is there any solar electric power plant having the concentrated photovoltaic modules?

The solar photovoltaic (PV) power plant uses commercial (non-concentrated) PV modules which are quite simple in design and reliable. They can work on fixed orientation and don't need the moving...

16 February 2021 3,796 9 View

Extraction of DNA from insect samples conserved in silica gel?

Hi everybody, can anybody suggest how to extract DNA from insect samples conserved in silica gel (in my case, mosquitoes)? Does anybody know a commericial kit which does that or a particular...

10 February 2021 3,392 4 View

How do I prepare and store insect samples for JH measurements?

I need to preserve termite samples for future quantification of juvenile hormone in their bodies. Or extract the Juvenile Hormone and keep that samples stored for future quantification. I may need...

09 February 2021 1,995 1 View