Hello everyboy, i need help How to do Rapid amplification of cDNA ends (RACE)?

Hello everyboy, i need help How to do Rapid amplification of cDNA ends (RACE)? i have got the sequence of two new genes in my species (Insect) now i want to do Rapid amplification of cDNA ends...

06 July 2018 8,762 2 View

Any suggestion on why different predicted band sizes appear for the same genes but different spp...?

Any suggestion on why different predicted band size appear for the same gene but different spp...? i am using primers for simple PCR amplification from previously publish paper. In published paper...

04 May 2018 6,868 6 View

How to make a lysis buffer for the extraction DNA from insects?

hello @ all researchers, i want to extract DNA from the midgut of insect so i need to know how to make a lysis buffer..? thanks

02 March 2018 4,870 3 View

protocol for lysis buffer and TE buffer for the extraction of DNA from insects .?

I need the protocol for lysis buffer and TE buffer for the extraction of DNA from insects Thanks

02 March 2018 994 4 View

Greeting,

I need this paper, Stuties on insecticide susceptibility of the beet armyworm fed on different host plants

06 July 2017 3,477 0 View

Hello, How do make a standard curve of alpha naphthol for estimating esterase enzyme activity? .

Please suggest how to dissolve the naphthol in buffer for making standard curve

06 July 2017 7,184 2 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

Any references about insects or animals that can damage or weathering of rock or fossil material?

Are there any references to insects or animals that can cause damage or weathering to the rock or fossil material? books or paper that would be nice. Thank you very much!

24 February 2021 6,805 3 View

Please help in identification of this insect?

This insect is minute in size around 1 mm and not clearly visible with naked eye.

22 February 2021 5,907 11 View

No title

22 February 2021 9,932 11 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

Is there a method for evaluating nanoparticle adhesion to epithelial tissue in vitro?

I want to measure how well nanoparticles can transfect cells based on NP adhesion properties. In the literature I find people do it in animal studies, and epithelial adhesion helps in transfection...

21 February 2021 7,791 3 View

How to select therorical or conceptual framework for any proposed qualitative research work?

Hi, I am conducting an exploratory research with qualitative in-depth interview approach to understand the impact of COVID_19 in health systems response to burn care in India. Since, this is a...

19 February 2021 4,562 4 View