hello everyone,
i am working on detoxification enzymes activities in insects. i need the protocol of in vitro metabolism of chlorantraniliprole insecticide..
Thanks
Hello everyboy, i need help How to do Rapid amplification of cDNA ends (RACE)? i have got the sequence of two new genes in my species (Insect) now i want to do Rapid amplification of cDNA ends...
06 July 2018 8,932 2 View
Any suggestion on why different predicted band size appear for the same gene but different spp...? i am using primers for simple PCR amplification from previously publish paper. In published paper...
04 May 2018 6,989 6 View
I need the protocol for lysis buffer and TE buffer for the extraction of DNA from insects Thanks
02 March 2018 1,095 4 View
hello @ all researchers, i want to extract DNA from the midgut of insect so i need to know how to make a lysis buffer..? thanks
02 March 2018 4,975 3 View
I need this paper, Stuties on insecticide susceptibility of the beet armyworm fed on different host plants
06 July 2017 3,577 0 View
Please suggest how to dissolve the naphthol in buffer for making standard curve
06 July 2017 7,376 2 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
I am new to research. The boiling point of triethyl amine (NEt3) is 89 degrees centigrade. I am skeptical that when I will let the hydrogen escape which is getting generated in situ, I will also...
21 July 2024 7,284 4 View
To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...
18 July 2024 9,177 2 View
I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...
17 July 2024 3,785 9 View
What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?
15 July 2024 1,598 3 View
Hello! everyone, I am trying to study in silico Protein-Protein and Protein-RNA interactions. Now, is there any tool with which I can identify the interacting amino acid residues or the...
14 July 2024 950 2 View
Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid...
14 July 2024 6,240 0 View