Any suggestion on why different predicted band sizes appear for the same genes but different spp...?

Any suggestion on why different predicted band size appear for the same gene but different spp...? i am using primers for simple PCR amplification from previously publish paper. In published paper...

04 May 2018 6,868 6 View

How to make a lysis buffer for the extraction DNA from insects?

hello @ all researchers, i want to extract DNA from the midgut of insect so i need to know how to make a lysis buffer..? thanks

02 March 2018 4,870 3 View

protocol for lysis buffer and TE buffer for the extraction of DNA from insects .?

I need the protocol for lysis buffer and TE buffer for the extraction of DNA from insects Thanks

02 March 2018 994 4 View

In vitro metabolism of chlorantraniliprole insecticide..?

hello everyone, i am working on detoxification enzymes activities in insects. i need the protocol of in vitro metabolism of chlorantraniliprole insecticide.. Thanks

02 March 2018 3,904 0 View

Greeting,

I need this paper, Stuties on insecticide susceptibility of the beet armyworm fed on different host plants

06 July 2017 3,477 0 View

Hello, How do make a standard curve of alpha naphthol for estimating esterase enzyme activity? .

Please suggest how to dissolve the naphthol in buffer for making standard curve

06 July 2017 7,184 2 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

QPCR reaction efficiency with odd results what does this indicate?

I am working on a CFX 96 machine to determine reaction efficiency of my gene of interest. After performing a serial dilution of my cDNA template, I was able to find great reaction efficiencies for...

23 February 2021 809 3 View

Could not amplify CD28 and CD3z ectodomain from cDNA of Jurkat Cells ?

For my experiments, I have to express ectodomains of human CD28 and CD3z. As a source, I extracted RNA from Jurkat cells and verified the quality of RNA on the gel. I, then, prepared cDNA using...

23 February 2021 7,051 4 View

Please help in identification of this insect?

This insect is minute in size around 1 mm and not clearly visible with naked eye.

22 February 2021 5,907 11 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View