Can somebody give me a proper protocol for isolating DNA from the pellet obtained after the harvested cells from cell culture done by cytogenetic method. The pellet is in 3:1 fixative (Clarke's solution)?
There may be many. Inorganic method and PCI method may be of your choice
I am aware of the PCI method from EDTA blood but in my case the WBC is suspended in methanol:glacial acetic acid (3:1). I obt ained it from the regular cytogenetics method. Can anybody tell me how to start from this step and continue with the PCI step?
Well, u need to centrifuge cell suspension and get rid of supernatant followed by consecutive washing of the pallet with PBS solution three to four times. PBS should be cold before use.
This link having article which might be useful. Because this article done the work exactly what you are looking...
http://www.ncbi.nlm.nih.gov/pubmed/24506057
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