Smita Kujur

4 Questions 14 Answers 0 Followers

Questions related from Smita Kujur

No amplification after PCR?

I successfully amplified my samples using Primer set 1 but when I'm amplifying the same samples using different Primer set, the genomic DNA is still stuck in the Wells and only ladder resolves. My...

07 July 2018 2,218 14 View

I used PCI method for DNA extraction, however, I did not obtain any DNA after adding chilled Iso 2 propanol.

This is my first time error, usually I never get this problem. I made 3M Sodium acetate but do not know how much micro litres to add. Can you let me know. Right now my sample with Iso 2 propanol...

06 June 2017 5,800 6 View

Can we use GR grade chemicals for Molecular Biology? or should I use only Molecular Biology grade? How about AR grade?

GR is usually used for cytogenetics but will it work for Molecular Biology? I have seen that the chemicals for Molecular work are different and not GR. What about AR grade? Can someone guide me?

06 June 2017 5,078 2 View

Protocol for isolating DNA from the pellet obtained after the harvested cells from cell culture done by cytogenetic method?

Can somebody give me a proper protocol for isolating DNA from the pellet obtained after the harvested cells from cell culture done by cytogenetic method. The pellet is in 3:1 fixative (Clarke's...

08 August 2016 1,246 5 View