I successfully amplified my samples using Primer set 1 but when I'm amplifying the same samples using different Primer set, the genomic DNA is still stuck in the Wells and only ladder resolves. My primers are new, buffers are fresh, DNA is 50 ng/ul and Primers are 10 pmol/ul. I even tried other PCR settings but nothing changed. No amplification. I used PCR conditions from other articles and still didn't help. Same samples are working well with other primers.

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