How best to thaw plasma samples for an immunoassay (cytokines)?

When I defrost my plasma samples from -80, I do so on ice, slowly. Then I centrifuge the samples. Upon centrifugation, the plasma samples will have a white sticky substance layer at the top, but...

30 July 2020 4,877 2 View

Different RNAdatase comparison?

Hi, I would like to compare my dataset generated with Illumina system with other datasets published and deposited on GEO. Is free software that allows me to do that? or just R software is enough?

29 September 2019 3,356 3 View

How can I clean glassware from TiO2 nanoparticles?

In my lab we've been synthesizing 10-20 nm TiO2 nanoparticles from Titanium Isopropoxide, and now the glassware, no matter how I clean it with solvents, is marked with a white patina. I could have...

05 May 2019 5,747 10 View

Influenza viruses plaque assay ?

Hi, I want a correct protocol for plaque assay with influenza viruses . I used MDCK CELLS ( 450 000 cell/well ) with different strains and different plates but i didn't see plaque formation but...

17 February 2019 5,686 4 View

Can protein folding affect antibody detection in a western blot?

I have problems with my western blots in that I get no signal. I have tried all the usual changing of ab concentration, different blocking etc but nothing is working. Is it possible that my...

21 October 2018 5,207 6 View

Have anybody here performed an abductive research strategy as a tool for textual interpretation of interviews?

Do you have any concrete suggestes about the methodological tools to handle abduction? I find that sorting an categorizing the data by coding is not satisfactory, and are looking for an...

01 August 2018 3,983 7 View

Is it possible to use regular Tris-Glycine Running buffer (SDS) on a NuPAGE Tris-Acetate 3-8% precast gel?

15 August 2017 8,144 3 View

THP 1 migration

Hi everyone! Do you have any suggestion regarding THP 1 migration assay? How many cells do you plate in a 24 well transwell with 8 um pore size? Incubation time? Thanks!

08 June 2017 6,333 2 View

What is the best method to concentrate PCR product after pooling several samples?

28 June 2015 5,116 11 View

Does anyone know of a good self-report questionnaire for locus of control?

Need it for a friends thesis which sets out to explore who succeeds in getting back to work after having been unemployed for a period of time.

20 December 2013 6,961 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View