How best to thaw plasma samples for an immunoassay (cytokines)?

When I defrost my plasma samples from -80, I do so on ice, slowly. Then I centrifuge the samples. Upon centrifugation, the plasma samples will have a white sticky substance layer at the top, but...

30 July 2020 4,877 2 View

Different RNAdatase comparison?

Hi, I would like to compare my dataset generated with Illumina system with other datasets published and deposited on GEO. Is free software that allows me to do that? or just R software is enough?

29 September 2019 3,356 3 View

How can I clean glassware from TiO2 nanoparticles?

In my lab we've been synthesizing 10-20 nm TiO2 nanoparticles from Titanium Isopropoxide, and now the glassware, no matter how I clean it with solvents, is marked with a white patina. I could have...

05 May 2019 5,747 10 View

Influenza viruses plaque assay ?

Hi, I want a correct protocol for plaque assay with influenza viruses . I used MDCK CELLS ( 450 000 cell/well ) with different strains and different plates but i didn't see plaque formation but...

17 February 2019 5,686 4 View

Can protein folding affect antibody detection in a western blot?

I have problems with my western blots in that I get no signal. I have tried all the usual changing of ab concentration, different blocking etc but nothing is working. Is it possible that my...

21 October 2018 5,207 6 View

Have anybody here performed an abductive research strategy as a tool for textual interpretation of interviews?

Do you have any concrete suggestes about the methodological tools to handle abduction? I find that sorting an categorizing the data by coding is not satisfactory, and are looking for an...

01 August 2018 3,983 7 View

Is it possible to use regular Tris-Glycine Running buffer (SDS) on a NuPAGE Tris-Acetate 3-8% precast gel?

15 August 2017 8,144 3 View

THP 1 migration

Hi everyone! Do you have any suggestion regarding THP 1 migration assay? How many cells do you plate in a 24 well transwell with 8 um pore size? Incubation time? Thanks!

08 June 2017 6,333 2 View

Protocol for F4/80 IHC on lung samples?

Hi everyone! I am trying to perform IHC for F4/80 (ab6640 abcam) on pfa fixed LUNG samples, but i'm not able to obtain a good staining. Do you know a good protocol? I usually use a concentration...

20 February 2017 2,652 2 View

What is the best method to concentrate PCR product after pooling several samples?

28 June 2015 5,116 11 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Which CpGs have a significant effect on gene expression?

It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...

20 February 2021 5,634 3 View

Found that there was a multiple band when amplifiying targeted gene ?

i few days ago amplified specific gene using PCR with specific primers, but there was a multiple band in electrophoregram i found. out of 10 sample amplified, there was multiple band and there was...

18 February 2021 5,131 5 View

Why is there no protein expression but antibiotic resistance in transduced A549 cells with pLVX-TetOne-Puro lentivirus?

Hi, I have been trying to transduce A549 and Calu-3 cells using the Lenti-X Tet-One inducible expression system (TakaraBio). My gene of interest is inserted in a pLVX-TetOne-Puro plasmid and has...

15 February 2021 8,462 3 View

Is there a way in which microglia can be extracted from mice without killing them ?

I'm aiming to use an adoptive cell transfer approach, with microglia in mice with ALS. So far all the protocols I have seen, require sacrificing the mouse. I'd like to re-infuse the microglia back...

11 February 2021 9,509 1 View