I recently noticed a higher variation in size among cells in my expi293/hek culture, and after some less than stellar protein yields from transfection, I've become concerned that my embryonic kidney cells are not the only denizens of my cell culture. Do the larger cells in the images appear to be contaminants either fungal or bacterial to anyone? Note that there are many cells roughly in between the size of the largest ones and the smaller ones pictured here.
Robert Adolf Brinzer
Just take a plate of LB agar and plate out 50μl and see if anything grows.
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Jan Philipp Dobert
Hi Adam,
I would not be worried. I see the same in my HEK 293F suspesion culture. I think these are just big cells.
Cheers,
Jan
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