FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

Fluorophore panel selection?

Hello, I'm trying to select fluorophores for a large panel of flow cytometry, so I'm wondering what is the maximum degree of overlap between two fluorophores that is considered acceptable? Thanks.

19 January 2021 3,475 3 View

Will TNF alpha receptor 1/2 (TNFR1 or TNFR2) be up- or down-regulated when endothelial cells are exposed to TNF alpha?

Excessive exposure to TNF alpha leads to inflammation, oxidative stress, and other pathological responses in cells like endothelial cells. When cells are treated with TNF alpha, will the receptors...

21 November 2020 4,039 3 View

About mechanical design?

Hello, I am a graduate student majoring in mechanical design. My mentor doesn't have a project now, I' m confused.Could you tell me what project everyone is studying? The scope is probably product...

08 October 2020 5,464 3 View

How best to thaw plasma samples for an immunoassay (cytokines)?

When I defrost my plasma samples from -80, I do so on ice, slowly. Then I centrifuge the samples. Upon centrifugation, the plasma samples will have a white sticky substance layer at the top, but...

30 July 2020 4,877 2 View

How to import files generated by Neper into ABAQUS for analysis?

I used Neper to generate the tessellation file and the meshing file, and assigned the crystal orientation. How to import these files into ABAQUS for crystal plastic finite element analysis(CPFEA,...

03 February 2020 7,608 8 View

Looking for a type of non-dividing primary cells to culture?

Dear community, Happy new year! I have a very rudimental question to bother you with. I'm interested in the principles of epigenetics. All the cells I'm familiar with are in cell cycle, such as...

31 December 2019 1,991 2 View

Is it possible to access a node or element set in a abaqus subroutine?

We define a set in abaqus preprocessor and we want to access it in a abaqus subroutine. Is this possible? What should I do?

29 November 2019 9,027 1 View

How to get the SNP flanking sequence(for Prunus mume, Japanese apricot)?

I already get SNP candidates for our target traits, then I would like use Real-time PCR based genotyping platform to check F1 population. For establishing the marker, the SNP flanking sequences...

09 November 2019 331 3 View

'Unable to start analysis job' error in ABAQUS CAE with a subroutine?

When I try to start a simulation in ABAQUS CAE with *.inp and *.f(umat file). The abaqus just prompt an error:"Unable to start analysis job" and stop without any debug message. The example files...

24 October 2019 1,436 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Can impedance of antenna be less than 50 ohms?

I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...

27 February 2021 4,218 7 View

Should we reduce the standard unit of length to 0.31951540592 meter ?

The reasoning behind this question is to align the ratios of the proton potential in energy per charge to the speed of light in distance per time. As these two dimensions appear to be related,...

27 February 2021 947 7 View

Is is possible to pole with corona poling pellets of the sample material instead of thin films?

As it is highly probable that pellets will have a higher degree of air gaps and voids than thin films, is it possible to pole the samples with corona poling nevertheless? Or is there the...

24 February 2021 1,349 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Helix antenna coupling problem?

Dealing with a helix antenna, made with flexible material and not rigid like using a PCB board or wire. At the beginning of the helix is too close to the ground plane, causing coupling. How can I...

21 February 2021 4,453 3 View

Can I arbitrarily take a cycle number of 40 for the gene that was not expressed at all in qPCR results calculation?

I ran RT-qPCR for a control and a treated group and it appears that my gene of interest was not expressed at all in any of the subjects in the control group. So I was wondering if I could...

20 February 2021 6,243 10 View

Can anyone help with an Accurate Diffuser Expansion Loss formula for rectangular shaped diffusers in turbulent flow regime?

I have tried Idelchik's Handbook of Hydraulic Resistance 4th Edition (2007 page 300) formulas and across some range the formulas are reported valid for, the law of conservation of energy is...

20 February 2021 2,504 1 View

Room Occupancy Calculation ?

15 February 2021 1,997 6 View