Can anyone tell me if their is any method or protocol to dry sds page gel after sample has been ran. The gel is to be stored for any further analysis.
You can use promega kit or thermo fisher kit for this.I have attached some useful links. Hope this will help.
http://www.protocol-online.org/biology-forums-2/posts/20185.html
https://www.thermofisher.com/np/en/home/references/protocols/proteins-expression-isolation-and-analysis/protein-gel-electrophoresis/gel-drying.html
1. If the gel has been subjected to standard staining and destaining techniques, soak the gel in water or a solution of 1% glycerol for 15-30 minutes. This removes any remaining acetic acid which may cause the gel to crack during drying. If the gel is to be dried directly after electrophoresis proceed directly to Step 2.
2. Place one of the plastic drying frames on a clean smooth surface.
3. Thoroughly moisten one cellulose sheet with water. Center and place the cellulose sheet over the frame. Remove any wrinkles so that the sheet lies flat with respect to the surface.
4. Wet the area of the sheet where the gel is to be placed with water.
5. Place the gel(s) to be dried on the wet sheet and position as desired. Remove any air bubbles that may be trapped between the gel and cellulose sheet.
6. Thoroughly moisten the second cellulose sheet. Slowly place this sheet over the gel(s) so that no air bubbles are trapped between the two sheets. Air bubbles can be removed by slowly pulling the second sheet back, rewetting the lower sheet, and then laying the second sheet down again.
7. Place the second drying frame directly over the first frame.
8. Carefully slide the frame assembly to the edge of the surface. Beginning at one edge of the frame assembly, grasp the edge of the exposed cellulose and gently pull it over the frame.
9. Clamp the folded edge of cellulose to the frame using the clamps provided. Continue this process around the frame.
10. Place the frame assembly in a horizontal position and allow it to dry in the hood over night.
Note: It is important to remove all air bubbles from between the two sheets. Air bubbles may cause cracking of the gel during drying.
Gradient Gels and High Percentage Acrylamide Gels (greater than 12% acrylamide):
1. Soak the gel in a solution of 30% methanol, 3% glycerol for 15-30 minutes. This will help minimize gel cracking during drying.
2. Continue with the procedure for SDS-polyacrylamide gels at Step 2.
You can use promega kit or thermo fisher kit for this.I have attached some useful links. Hope this will help.
http://www.protocol-online.org/biology-forums-2/posts/20185.html
https://www.thermofisher.com/np/en/home/references/protocols/proteins-expression-isolation-and-analysis/protein-gel-electrophoresis/gel-drying.html
1. If the gel has been subjected to standard staining and destaining techniques, soak the gel in water or a solution of 1% glycerol for 15-30 minutes. This removes any remaining acetic acid which may cause the gel to crack during drying. If the gel is to be dried directly after electrophoresis proceed directly to Step 2.
2. Place one of the plastic drying frames on a clean smooth surface.
3. Thoroughly moisten one cellulose sheet with water. Center and place the cellulose sheet over the frame. Remove any wrinkles so that the sheet lies flat with respect to the surface.
4. Wet the area of the sheet where the gel is to be placed with water.
5. Place the gel(s) to be dried on the wet sheet and position as desired. Remove any air bubbles that may be trapped between the gel and cellulose sheet.
6. Thoroughly moisten the second cellulose sheet. Slowly place this sheet over the gel(s) so that no air bubbles are trapped between the two sheets. Air bubbles can be removed by slowly pulling the second sheet back, rewetting the lower sheet, and then laying the second sheet down again.
7. Place the second drying frame directly over the first frame.
8. Carefully slide the frame assembly to the edge of the surface. Beginning at one edge of the frame assembly, grasp the edge of the exposed cellulose and gently pull it over the frame.
9. Clamp the folded edge of cellulose to the frame using the clamps provided. Continue this process around the frame.
10. Place the frame assembly in a horizontal position and allow it to dry in the hood over night.
Note: It is important to remove all air bubbles from between the two sheets. Air bubbles may cause cracking of the gel during drying.
Gradient Gels and High Percentage Acrylamide Gels (greater than 12% acrylamide):
1. Soak the gel in a solution of 30% methanol, 3% glycerol for 15-30 minutes. This will help minimize gel cracking during drying.
2. Continue with the procedure for SDS-polyacrylamide gels at Step 2.
If the equipment is available to you, there is a device called a gel dryer that uses a combination of heat and vacuum to dry gels onto filter paper. It is faster than the air drying method described by Hriush.
An example of such an instrument can be found here:
http://www.bio-rad.com/en-us/category/protein-electrophoresis-blotting/gel-drying-equipment
How long do you want to store the gel? We usually seal them in 0,1% acetic acid at 4°C and they can be used for downstream LC-MS/MS for longer period of time.
Use method suggested by Dr Adam B Shapiro... work very well.. good luck..
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