Tim Schelfhorst

5 Questions 33 Answers 0 Followers

Questions related from Tim Schelfhorst

White spots on blot, no bubbles in transfer?

Recently I keep getting similar issues with 4G10 platinum antibodies. When I try to look for total phosphorylation I keep getting these kinds of white spots on the blot. At first I thought these...

09 September 2016 986 6 View

Is it possible to reduce the protein already in a western blot?

I've blotted several samples under non-reducing conditions(because I have 2 non-reducing antibodies, CD63 & CD81) but these are both negative. Now I want to try staining with ALIX, but for...

10 October 2015 3,945 7 View

I am looking for a stringent wash buffer that does not lysate exosomes?

Hi, I have isolated exosomes from plasma, the exosomes are in some sort of protein aggregate. Is there anyway to wash away other plasma proteins (albumins, fibrinogens etc.) without lysing the...

12 December 2014 9,924 4 View

Does anyone have an idea, how long it will take for a single mcf7 cell to grow into a clump, or double for that matter?

Does anyone have an idea, how long it will take for a single mcf7 cell to grow into a clump, or double for that matter? I want to culture from single cells.

10 October 2013 6,070 13 View

Can someone recommend a 25mm opening culture vessel which is small?

I'm looking for a culture vessel of some kind, with a opening of at least 25mm, and dimensions closest to 60mmx30mmx5mm. Preferably not round but flat. The only option I now have is a T150 flask...

10 October 2013 9,948 9 View