15 Questions 31 Answers 0 Followers
Questions related from Shubhashish Chakraborty
Hello. I want to crystallize kinase domain of a protein in active form. The active form of the domain has specifically phosphorylated tyrosine residues. However, I am not sure if I can do so in a...
02 February 2020 7,538 5 View
Is their any online bioinformatic tool available through which I can understand or know the interacting partners of a kinase domain of the protein I am studying? I wish to study interactome of...
07 July 2019 6,093 6 View
I have purified a protein, however I am getting double band on SDS-PAGE. Even after FPLC the double band doesn't resolve and become more prominent. I have checked through Mass spec that both the...
07 July 2019 6,720 10 View
I am purifying a protein expressed in pLysS cells. The construct is in pET28 vector and having His tag with TEV cleavage site . Now when I ran the SDS-PAGE gels after TEV cleavage and...
04 April 2019 7,569 16 View
In my construct I have two His tags, one at N-term and another at C-term. There is a TEV site between the N-term His tag and my gene but no cleavage site for the C-term. So, do I need to add...
04 April 2019 2,322 8 View
Is it that vectors with T7 promoter do not grow/transform well in rosetta or codon cells?
03 March 2019 1,010 3 View
I am not getting colonies when I had transformed my construct in rosetta 2 cells whereas transformation worked in Bl21-pLyse cells. I had used the same heat shock protocol for both. The one change...
02 February 2019 2,213 11 View
Can any one provide me appropriate protocol for preparing crude mouse tissue lysate for western blotting. The protein of interest resides both in cytoplasm and nucleus so which lysis buffer would...
02 February 2017 5,358 6 View
I want to study genetic relatedness among fresh water fishes, so which molecular marker would be best for this study?
08 August 2016 6,231 2 View
Does any one have any protocol or assays by which I could study signalling profile of tor gene during various stress conditions in yeast?
07 July 2016 9,951 0 View
I want to test expression of one of the protein in clinical samples of oral cancer. So can I use cheek cells as control or I need to derive some other tissue from mouth of normal individual?
07 July 2016 1,330 4 View
Can anyone refer me paper related to TOR1/2 gene expression analysis in yeast.
06 June 2016 4,276 2 View
Can any one give me a detailed protocol for 16s rRNA PCR using 28F and 1492R primers. I want to know the concentration of working solution of the two primers and anneling temperature in particular.
06 June 2016 8,681 5 View
Can anyone tell me if their is any method or protocol to dry sds page gel after sample has been ran. The gel is to be stored for any further analysis.
06 June 2016 3,271 5 View
Hi all. I want to insert an enterokinase site in my plasmid using PCR mediated site directed mutagenesis, so is thier any standard protocol for this. My insert size is 14nt and plasmid 4.2kb
06 June 2016 1,342 5 View
