Fraction collector Frac-920 not working with AKTA?

Hi, I routinely use AKTA connected to Fraction Collector-920 for collecting proteins while doing protein purification. However, recently I have started seeing some errors on our Fraction...

27 February 2020 9,025 4 View

No colonies from NEB high-efficiency chemically competent cells?

Hi all, I am doing a few site-directed mutagenesis reactions and after digestion of the parent plasmid using Dpn1, I add the complete 50 uL reaction volume to 50 uL of NEB chemically competent...

01 November 2019 3,085 3 View

Low plasmid miniprep yields?

Hi, I have been working on plasmid minipreps using Qiagen miniprep kit and I have run into weird problems of low plasmid yields. When we purchased a new kit, then my plasmid concentrations were...

12 March 2019 9,028 3 View

What could be the probable mechanism underlying the reduction in body weight of L-NAME treated rats?

What could be the probable mechanism underlying the reduction in body weight of L-NAME treated rats when compared to Control rats?

14 September 2018 6,964 2 View

How to prepare simulated distillery wastewater in laboratory?

I want to check the bio-remediation of distillery wastewater.

10 June 2018 1,340 3 View

Does anyone have expertise using Getis-Ord hotspot analysis?

14 March 2017 541 2 View

Calculating nanomoles with knowing only conc and OD?

Hi All, I am wondering if we can calculate the nanomoles of RNAi, knowing only details of its ug/ul concentration and OD values; though no information of basepair is available? please suggest! Best,

02 February 2017 432 3 View

Is there any empirical relation to deduce mineral N in the soil layer on the basis of Available nitrogen?

Mineral N (nitrate and ammonical form) is highly dynamic, and is generally not measured by most of the researchers, who most of the time take total N or available N in consideration for evaluating...

28 January 2016 6,998 5 View

Several soil quality indices reported in literature, but effective operational soil health service missing. Please suggest the ways.

1. Lot of information on soil test values (spatio-temporal)) available 2. Soil quality indices e.g. SQI by Ratan Lal et al, Cornell soil health index, STCR network ar Indian Institute of Soil...

21 January 2016 8,570 26 View

What is the process of converting HB (harwell boeing) matrix to sparse matrix?

To get the stiffness matrix of a FE model from ANSYS to MATLAB I converted it into HB format. Now I want to convert it into sparse format using MATLAB. Is there any program in MATLAB to do so. If...

13 January 2015 369 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to evaluate unsupervised clustering metrics when some elements do not have a cluster label in the result?

I run algorithms like HDBSCAN and MeanShift, and compare them with K-means and GaussianMixture. The first two algorithms do not return cluster labels for some elements in the dataset. My question...

27 February 2021 428 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View