Fraction collector Frac-920 not working with AKTA?

Hi, I routinely use AKTA connected to Fraction Collector-920 for collecting proteins while doing protein purification. However, recently I have started seeing some errors on our Fraction...

27 February 2020 9,025 4 View

No colonies from NEB high-efficiency chemically competent cells?

Hi all, I am doing a few site-directed mutagenesis reactions and after digestion of the parent plasmid using Dpn1, I add the complete 50 uL reaction volume to 50 uL of NEB chemically competent...

01 November 2019 3,085 3 View

Low plasmid miniprep yields?

Hi, I have been working on plasmid minipreps using Qiagen miniprep kit and I have run into weird problems of low plasmid yields. When we purchased a new kit, then my plasmid concentrations were...

12 March 2019 9,028 3 View

What could be the probable mechanism underlying the reduction in body weight of L-NAME treated rats?

What could be the probable mechanism underlying the reduction in body weight of L-NAME treated rats when compared to Control rats?

14 September 2018 6,964 2 View

How to prepare simulated distillery wastewater in laboratory?

I want to check the bio-remediation of distillery wastewater.

10 June 2018 1,340 3 View

Why is my P3HT TFT not turning on?

When measuring the devices, I have found that the gate is connected however the device is not turning on i.e the Id-Vg curve is flat. Any suggestions as to why this might be?

28 July 2017 7,741 2 View

Does anyone have expertise using Getis-Ord hotspot analysis?

14 March 2017 541 2 View

Calculating nanomoles with knowing only conc and OD?

Hi All, I am wondering if we can calculate the nanomoles of RNAi, knowing only details of its ug/ul concentration and OD values; though no information of basepair is available? please suggest! Best,

02 February 2017 432 3 View

Any good and working protocol for DNA isolation from somatic cells of milk?

Factors affecting somatic cell count in milk.

21 July 2016 7,936 3 View

Can patients suffering from diabetes have balance related problems well before peripheral neuropathy is detected clinically?

i wish to know that as diabetics have gait alterations well before PN is detected can they have balance problems also. i am not able to search an article on it.

20 July 2016 4,942 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Are there any open-source datasets containing physiological information about exercises to be used for an OR-model?

I have just recently started a new "weekend project" in addition to my master's studies and I am looking for a data-set. I would like to use some Operations Research to design an optimal gym...

22 February 2021 8,023 1 View

Why the nucleic acid gel melted after running for 30min?

Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...

22 February 2021 9,896 3 View

Staining methods for skeletal muscle fiber typing?

does anyone know the best method to differentiate and quantify rapid fatiguing and fatigue resistant muscle fibers? the old method form the 1980's uses ATP staining which is quite labor intense?

21 February 2021 6,483 3 View