Fraction collector Frac-920 not working with AKTA?

Hi, I routinely use AKTA connected to Fraction Collector-920 for collecting proteins while doing protein purification. However, recently I have started seeing some errors on our Fraction...

27 February 2020 9,025 4 View

No colonies from NEB high-efficiency chemically competent cells?

Hi all, I am doing a few site-directed mutagenesis reactions and after digestion of the parent plasmid using Dpn1, I add the complete 50 uL reaction volume to 50 uL of NEB chemically competent...

01 November 2019 3,085 3 View

Low plasmid miniprep yields?

Hi, I have been working on plasmid minipreps using Qiagen miniprep kit and I have run into weird problems of low plasmid yields. When we purchased a new kit, then my plasmid concentrations were...

12 March 2019 9,028 3 View

What could be the probable mechanism underlying the reduction in body weight of L-NAME treated rats?

What could be the probable mechanism underlying the reduction in body weight of L-NAME treated rats when compared to Control rats?

14 September 2018 6,964 2 View

How to prepare simulated distillery wastewater in laboratory?

I want to check the bio-remediation of distillery wastewater.

10 June 2018 1,340 3 View

Does anyone have expertise using Getis-Ord hotspot analysis?

14 March 2017 541 2 View

Calculating nanomoles with knowing only conc and OD?

Hi All, I am wondering if we can calculate the nanomoles of RNAi, knowing only details of its ug/ul concentration and OD values; though no information of basepair is available? please suggest! Best,

02 February 2017 432 3 View

Is there any empirical relation to deduce mineral N in the soil layer on the basis of Available nitrogen?

Mineral N (nitrate and ammonical form) is highly dynamic, and is generally not measured by most of the researchers, who most of the time take total N or available N in consideration for evaluating...

28 January 2016 6,998 5 View

Several soil quality indices reported in literature, but effective operational soil health service missing. Please suggest the ways.

1. Lot of information on soil test values (spatio-temporal)) available 2. Soil quality indices e.g. SQI by Ratan Lal et al, Cornell soil health index, STCR network ar Indian Institute of Soil...

21 January 2016 8,570 26 View

What is the process of converting HB (harwell boeing) matrix to sparse matrix?

To get the stiffness matrix of a FE model from ANSYS to MATLAB I converted it into HB format. Now I want to convert it into sparse format using MATLAB. Is there any program in MATLAB to do so. If...

13 January 2015 369 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View