7 Questions 11 Answers 0 Followers
Questions related from Prakriti Kalra
Hi all, I am performing a protein (15 kDa)-DNA (41 bp ds DNA) co-crystallization and have some questions: 1. The DNA has a mid-micromolar affinity for the protein. So, what should be the...
28 October 2020 6,321 0 View
Hi, I routinely use AKTA connected to Fraction Collector-920 for collecting proteins while doing protein purification. However, recently I have started seeing some errors on our Fraction...
28 February 2020 9,240 4 View
Hi all, I am doing a few site-directed mutagenesis reactions and after digestion of the parent plasmid using Dpn1, I add the complete 50 uL reaction volume to 50 uL of NEB chemically competent...
02 November 2019 3,220 3 View
Hi all, I do the expression of wt unlabeled and fluorinated domains of euakaryotic proteins in E.Coli BL21(de3) cells. Over the past few months, I have been noticing that during our FPLC...
30 May 2019 8,120 5 View
Hi, I have been working on plasmid minipreps using Qiagen miniprep kit and I have run into weird problems of low plasmid yields. When we purchased a new kit, then my plasmid concentrations were...
13 March 2019 9,173 3 View
Hi everyone, I am working on studying proteins labeled with fluorine tags via 19F NMR. I had a question about the free induction decay data that I have been getting. I am attaching a picture of...
27 February 2019 8,550 0 View
Hello, I am expressing wt. BRD4-D1 (only first bromodomain of BRD4 protein) using BL21 cells. During cell lysis with lysis buffer and lysozyme, I add a saturated solution of PMSF in 1 mL of...
10 August 2018 9,251 8 View
