Problems with PC12 cell culture?

01 January 2014 1 4K Report

Hi.

I am working with a PC12 cell line. Most of cells in T-25 flask are round, dark green. What does this mean? Have my cells died or are in shock? How should I work with them?

Trung Kien Nguyen

Your culture media should content Horse serum (10%) and FBS (5%). For protection of Bacteria infection, It should content antibiotic also.

You can have more detail information at ATCC or Sigma.

About you problem, it very difficult to help with very few information you had given.

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