Could you suggest another procedure?
You can use High pure Templat PCR Kit, if you want protocol I can send it to you, I already works in brucella and I extracted DNA from serum many time before
I want to fuse a protein (830 aa) with RFP. I have some linker sequences, but I'm not sure which linker is suitable? short or long? which sequence? this protein is a heat shock one and expresses...
05 June 2016 3,215 4 View
Hello I want to investigate the effect of high temperature on ROS production in yeast. I have yeast cells which grow at 30 degree as a control and experiment cells grown at high temperature.I used...
03 April 2016 6,374 6 View
Hi. I did real-time PCR for 50% samples without any problem. I had just one great pick for my gene. No primer dimer. but suddenly, for other 50% samples I got primer dimer pick as well as my gene...
31 December 2015 4,397 7 View
How long after seeding of cell line, should I do serum starvation or serum 4%? 24 h or less? How long should it last (before treatment) or serum 4%? 24 h or less? i i want to do serum starvation...
11 December 2014 3,961 3 View
I have to select one of them (no other cell lines), because my study is about rats and there are only these cell lines of brain in my country.
08 September 2014 5,149 1 View
Hi. I want to investigate the effect of an herbal drug for memory genes. I selected PC12 cell line. This cell line differentiates neuron-like cells after NGF treatment. Because the NGF is very...
06 July 2014 9,922 4 View
I want to treat my PC12 cell line with one drug, but the drug is insoluble in water and DMSO. I dissolved the drug in DMSO and media, but crystal was formed. What solvent do you suggest? What...
01 February 2014 4,384 4 View
Hi. I am working with a PC12 cell line. Most of cells in T-25 flask are round, dark green. What does this mean? Have my cells died or are in shock? How should I work with them?
31 December 2013 4,004 1 View
31 December 2013 4,399 4 View
I want to extract brucella DNA from human serum. I have selected the "salting out" procedure, but I don't have a protocol for this. Could you suggest another procedure?
31 December 2013 8,739 4 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...
26 February 2021 5,029 2 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...
25 February 2021 1,660 3 View
Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...
25 February 2021 3,612 3 View
Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...
24 February 2021 5,749 3 View
I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...
24 February 2021 4,397 3 View