as Jebelli

11 Questions 8 Answers 0 Followers

Questions related from as Jebelli

Which is the best linker for fusion protein?

I want to fuse a protein (830 aa) with RFP. I have some linker sequences, but I'm not sure which linker is suitable? short or long? which sequence? this protein is a heat shock one and expresses...

06 June 2016 3,324 4 View

Why high ROS signal in flowcytometry and fluorescence microscope?

Hello I want to investigate the effect of high temperature on ROS production in yeast. I have yeast cells which grow at 30 degree as a control and experiment cells grown at high temperature.I used...

04 April 2016 6,424 6 View

Can anyone assist me in avoiding primer dimer in real-time PCR?

Hi. I did real-time PCR for 50% samples without any problem. I had just one great pick for my gene. No primer dimer. but suddenly, for other 50% samples I got primer dimer pick as well as my gene...

01 January 2016 4,454 7 View

Serum starvation in cell line treatment?

How long after seeding of cell line, should I do serum starvation or serum 4%? 24 h or less? How long should it last (before treatment) or serum 4%? 24 h or less? i i want to do serum starvation...

12 December 2014 3,996 3 View

Which cell line of C6, B65 and B92 is appropriate for study of memory gene expression after treatment with a drug?

I have to select one of them (no other cell lines), because my study is about rats and there are only these cell lines of brain in my country.

09 September 2014 5,190 1 View

Which neuronal cell line is a appropriate selection?

Hi. I want to investigate the effect of an herbal drug for memory genes. I selected PC12 cell line. This cell line differentiates neuron-like cells after NGF treatment. Because the NGF is very...

07 July 2014 9,970 4 View

Any suggested solvents for drug dissolution?

I want to treat my PC12 cell line with one drug, but the drug is insoluble in water and DMSO. I dissolved the drug in DMSO and media, but crystal was formed. What solvent do you suggest? What...

02 February 2014 4,436 4 View

What is an appropriate solvent for drug dissolution?

I want to treat my PC12 cell line with one drug, but the drug is insoluble in water and DMSO. I dissolved the drug in DMSO and media, but crystal was formed. What solvent do you suggest? What...

01 January 2014 4,444 4 View

How to extract brucella DNA from human serum?

I want to extract brucella DNA from human serum. I have selected the "salting out" procedure, but I don't have a protocol for this. Could you suggest another procedure?

01 January 2014 8,808 4 View

Problems with PC12 cell culture?

Hi. I am working with a PC12 cell line. Most of cells in T-25 flask are round, dark green. What does this mean? Have my cells died or are in shock? How should I work with them?

01 January 2014 4,066 1 View

I want to extract brucella DNA from human serum. I selected the "salting out" procedure, but I don't have a protocol for serum.

Could you suggest another procedure?

02 February 2013 6,167 1 View