I want to treat my PC12 cell line with one drug, but the drug is insoluble in water and DMSO. I dissolved the drug in DMSO and media, but crystal was formed. What solvent do you suggest? What ratio should I use?
I work with Olibanum. I prepared aqueous and alcohol extraction of Olibanum and then I dried these extractions according to protocol. I don't buy Olibanum from any company (this is a traditional material) and I don't have any data sheet of this drug. I want to prepare a stock solution (for example 100X) for these extractions and then used them for cell line treatment, but I can't dissolve them in DMSO.
Maybe you could try acetic acid; I recomend to use a small amount of concentrated solvent, and then use distilled water to decrease solvent concentration. Good luck
This is a complex plant material and you may simply not be able to find a solvent that will dissolve all its components ? I would suggest a sequential treatment with several of the solvents suggested above until you have dissolved all the various components. You will need to test the different solvent fractions separately and remember to do the appropriate solvent controls. Using this method you should be able to use the cell culture results to identify the solvent that is going to give you the active fraction that you are after. I think it will be more useful than spending a lot of time looking for the ultimate solvent.
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